Department of Infectious Diseases, The First Affiliated Hospital, College of Clinical Medicine, Henan University of Science and Technology, Luoyang, 471000, China.
Department of Neurosurgery, Shaoxing People's Hospital, Shaoxing Hospital, Zhejiang University School of Medicine, Shaoxing 312000, China.
Sci Total Environ. 2023 Jul 15;882:163600. doi: 10.1016/j.scitotenv.2023.163600. Epub 2023 Apr 20.
MCR-positive Escherichia coli (MCRPEC) have been reported in humans worldwide. The high prevalence of mcr-1 poses clinical and environmental risks due to its diverse genetic mechanisms. Given the vital role of animals and the environment in the spread of antibiotic resistance, a "One Health" perspective should be taken when addressing antimicrobial resistance issues. This study conducted a prospective study in six farms (located in Jiaxing City, Zhejiang province, China) in 2019. MCRPEC strains were screened from samples of different sources. The molecular epidemiological surveys and transmission potential were investigated by whole-genome sequencing and phylogenetic analysis. MCRPEC were detected in different farms with various sources. Sequence type complex 10 was dominant and distributed widely in multiple sources. Core-genome multilocus sequence type (cgMLST) analysis indicated that clonal transmission could occur within and between farms. In addition, mcr-1 genes with different locations showed different transmission tendencies. The study indicated that interspecies and cross-regional transmission of MCRPEC could occur between different sectors in farms. Further surveillance and research of non-clinical MCRPEC strains are necessary to reduce the threat of MCRPEC.
MCR-阳性大肠杆菌（MCRPEC）已在全球范围内的人类中被报道。由于其多样化的遗传机制，mcr-1 的高流行率带来了临床和环境风险。鉴于动物和环境在抗生素耐药性传播中的重要作用，在解决抗微生物药物耐药性问题时应采取“同一健康”的观点。本研究于 2019 年在 6 个农场（位于中国浙江省嘉兴市）进行了一项前瞻性研究。从不同来源的样本中筛选出 MCRPEC 菌株。通过全基因组测序和系统发育分析，对分子流行病学调查和传播潜力进行了研究。在不同的农场中检测到了不同来源的 MCRPEC。序列型复合体 10 是优势种群，分布广泛，存在于多种来源中。核心基因组多位点序列型（cgMLST）分析表明，种内和种间的克隆传播可能在农场内部和农场之间发生。此外，具有不同位置的 mcr-1 基因表现出不同的传播趋势。研究表明，MCRPEC 可能在农场的不同部门之间发生种间和跨区域传播。进一步监测和研究非临床 MCRPEC 菌株对于降低 MCRPEC 的威胁是必要的。
