Tsyganova Anna V, Gorshkov Artemii P, Vorobiev Maxim G, Tikhonovich Igor A, Brewin Nicholas J, Tsyganov Viktor E
Laboratory of Molecular and Cell Biology, All-Russia Research Institute for Agricultural Microbiology, 196608 Saint Petersburg, Russia.
Research Park, Saint Petersburg State University, 199034 Saint Petersburg, Russia.
Plants (Basel). 2024 Oct 18;13(20):2923. doi: 10.3390/plants13202923.
Hydrogen peroxide (HO) in plants is produced in relatively large amounts and plays a universal role in plant defense and physiological responses, including the regulation of growth and development. In the -legume symbiosis, hydrogen peroxide plays an important signaling role throughout the development of this interaction. In the functioning nodule, HO has been shown to be involved in bacterial differentiation into the symbiotic form and in nodule senescence. In this study, the pattern of HO accumulation in pea ( L.) wild-type and mutant nodules blocked at different stages of the infection process was analyzed using a cytochemical reaction with cerium chloride. The observed dynamics of HO deposition in the infection thread walls indicated that the distribution of HO was apparently related to the stiffness of the infection thread wall. The dynamics of HO accumulation was traced, and its patterns in different nodule zones were determined in order to investigate the relationship of HO localization and distribution with the stages of symbiotic nodule development in . The patterns of HO localization in different zones of the indeterminate nodule have been partially confirmed by comparative analysis on mutant genotypes.
植物体内的过氧化氢（H₂O₂）产量相对较高，在植物防御和生理反应中发挥着普遍作用，包括对生长发育的调控。在豆科植物共生过程中，过氧化氢在这种相互作用的整个发育过程中发挥着重要的信号传导作用。在功能正常的根瘤中，已证明H₂O₂参与细菌分化为共生形态以及根瘤衰老过程。在本研究中，使用氯化铈的细胞化学反应分析了豌豆（Pisum sativum L.）野生型和在感染过程不同阶段受阻的突变体根瘤中H₂O₂的积累模式。观察到感染丝壁中H₂O₂沉积的动态变化，表明H₂O₂的分布显然与感染丝壁的硬度有关。追踪了H₂O₂积累的动态变化，并确定了其在不同根瘤区域的模式，以研究H₂O₂的定位和分布与豌豆共生根瘤发育阶段的关系。通过对突变体基因型的比较分析，部分证实了不定根瘤不同区域中H₂O₂的定位模式。
