Tukhovskaya Elena A, Palikova Yulia A, Severyukhina Mariya S, Ismailova Alina M, Palikov Victor A, Slashcheva Gulsara A, Borozdina Natalya A, Mikhaylov Evgeniy S, Kravchenko Irina N, Kazakov Vitaly A, Kazakova Ekaterina N, Kalabina Elena A, Rasskazova Ekaterina A, Shinelev Maxim V, Rzhevsky Dmitry I, Rykov Vladimir A, Dyachenko Igor A, Murashev Arkady N
Shemyakin-Ovchinnicov Institute of Bioorganic Chemistry (Branch), Russian Academy of Sciences, Prospekt Nauki, 6, Pushchino 142290, Russia.
Pharmaceuticals (Basel). 2024 Oct 11;17(10):1360. doi: 10.3390/ph17101360.
: Intratracheal administration of bleomycin (BLM) to laboratory rodents is a standard, widely used technique used to model pulmonary fibrosis (PF). BLM, as a modeling agent, is produced mainly in the form of two salts-sulfate and chloride. We compared the results of modeling PF in SD rats by intratracheal administration of BLM sulfate and BLM chloride. : Healthy mature male SD rats were used. PF was modeled by intratracheal administration of BLM sulfate and BLM chloride at a dose of 3 mg/kg. The criteria for the development of PF included body weight gain, changes in respiratory parameters, relative lung weight, cellular composition of broncho-alveolar fluid (BALF), histological assessment of the severity of PF with trichrome Masson staining. : Intratracheal administration of both BLM salts led to the development of pronounced PF, which was determined by changes in all of the measured parameters relative to control animals. There were no significant differences between the BLM sulfate and BLM chloride groups in body weight gain, hydroxyproline content, and histological evaluation. However, significant differences were identified in the cellular composition of BALF-a significant increase in alveolar macrophages and neutrophils levels in animals treated with BLM sulfate. : Intratracheal administration of both BLM salts led to the development of severe PF; however, the inflammatory process in animals receiving BLM sulfate was more pronounced and prolonged than in animals receiving BLM chloride, which in the former, when observed more than 21 days after modeling, can lead to more severe PF.
向实验啮齿动物气管内注射博来霉素（BLM）是一种用于模拟肺纤维化（PF）的标准且广泛使用的技术。作为建模剂的BLM主要以两种盐的形式产生——硫酸盐和氯化物。我们比较了通过气管内注射硫酸博来霉素和氯化博来霉素在SD大鼠中模拟PF的结果。：使用健康成熟的雄性SD大鼠。通过气管内注射剂量为3mg/kg的硫酸博来霉素和氯化博来霉素来模拟PF。PF发展的标准包括体重增加、呼吸参数变化、相对肺重量、支气管肺泡灌洗液（BALF）的细胞组成、用三色马松染色对PF严重程度进行组织学评估。：气管内注射两种BLM盐均导致明显的PF发展，这由相对于对照动物的所有测量参数的变化确定。硫酸博来霉素组和氯化博来霉素组在体重增加、羟脯氨酸含量和组织学评估方面没有显著差异。然而，在BALF的细胞组成中发现了显著差异——硫酸博来霉素处理的动物中肺泡巨噬细胞和中性粒细胞水平显著增加。：气管内注射两种BLM盐均导致严重PF的发展；然而，接受硫酸博来霉素的动物中的炎症过程比接受氯化博来霉素的动物更明显且持续时间更长，在前者中，建模后观察超过21天时，可能导致更严重的PF。
