Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wolczanska 171/173, 90-530 Lodz, Poland.
Institute of Food Technology and Analysis, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland.
Molecules. 2024 Oct 17;29(20):4922. doi: 10.3390/molecules29204922.
Some potentially probiotic strains of lactic acid bacteria (LAB) and yeast that inhabit the digestive tract of humans are known to detoxify xenobiotics, including acrylamide (AA). The objective of the subsequent research was to evaluate the AA-detoxification capability of LAB and yeast isolated from various sources. Namely, the effect of AA was tested on the growth of LAB and yeast strains, as well in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Subsequently, the AA-binding ability of LAB and yeast was investigated in various environments, including the pH, incubation temperature, cell density, and with inanimate cells. The ability of selected LAB and yeast to reduce the genotoxicity of AA was tested on Caco-2 and Hep-G2 cell lines. The results showed that all tested strains exhibited strong resistance to AA at concentrations of 5, 10, and 50 µg/mL. Also, AA was detected in the intracellular and membrane extracts of tested strains. The most effective binding strain was 16 at pH = 5, cell density = 10 CFU/mL, and incubation temperature = 37 °C (87.6% of AA removed). Additionally, all tested strains reduced the genotoxicity of AA, with the greatest reduction observed at the highest concentration of 50 µg/mL. The phenomena of detoxification by potentially probiotic strains could reduce the toxic and harmful effects of AA exposure to humans every day.
一些存在于人类消化道中的潜在益生菌乳酸杆菌 (LAB) 和酵母菌株已知能够解毒包括丙烯酰胺 (AA) 在内的外来化合物。随后的研究旨在评估从不同来源分离出的 LAB 和酵母对 AA 的解毒能力。即，通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐 (MTT) 测定法测试 AA 对 LAB 和酵母菌株生长的影响。随后，在不同环境中研究了 LAB 和酵母对 AA 的结合能力，包括 pH 值、孵育温度、细胞密度以及与无生命细胞的结合能力。选择的 LAB 和酵母减少 AA 遗传毒性的能力在 Caco-2 和 Hep-G2 细胞系上进行了测试。结果表明，所有测试菌株在 5、10 和 50 µg/mL 的浓度下均对 AA 表现出很强的抗性。此外，在测试菌株的细胞内和膜提取物中检测到 AA。在 pH = 5、细胞密度 = 10 CFU/mL 和孵育温度 = 37°C 时，结合能力最强的菌株为 16（去除了 87.6%的 AA）。此外，所有测试菌株均降低了 AA 的遗传毒性，在 50 µg/mL 的最高浓度下观察到最大的降低效果。潜在益生菌菌株的解毒现象可以减少人们每天接触 AA 时产生的毒性和有害影响。
