Assessment of the genotoxic potential of unleaded gasoline and 2,2,4-trimethylpentane in human lymphoblasts in vitro.

The potential of unleaded gasoline and 2,2,4-trimethylpentane to induce gene locus mutation and sister-chromatid exchange in human lymphoblasts in vitro was determined. Neither unleaded gasoline, at its maximum tolerated concentration in the medium, nor 2,2,4-trimethylpentane, at its limit of solubility, induced mutation at the thymidine kinase locus. Negative results were seen both in the presence and absence of a rat liver homogenate metabolizing system. Sister-chromatid exchange analyses of the cultures treated with unleaded gasoline and 2,2,4-trimethylpentane were also negative. Therefore the carcinogenicity and nephrotoxicity of unleaded gasoline and 2,2,4-trimethylpentane observed in vivo are not correlated with any marked genotoxicity in these in vitro tests.