Mason R W, Hogg S J, Edwards I R
Toxicology. 1986 Feb;38(2):219-26. doi: 10.1016/0300-483x(86)90123-x.
The urinary and renal distribution of Pt following the administration of cis-dichlorodiammine platinum II (cisplatin) to adult male Wistar rats was studied by gel filtration and ion exchange chromatography. Several low molecular weight (LMW) Pt-containing fractions with the same chromatographic properties as those found in urine incubated with cisplatin are present in the kidney cytosol within 15 min of the administration of cisplatin. The concentration of Pt in these fractions decreases rapidly and after 4 h most of the Pt in the kidney cytosol is in a high molecular weight (HMW) fraction which contains 2 subfractions. The smaller fraction (mol. wt. = 20 000), but not the larger (mol. wt greater than 250 000), is also present in the urine of the cisplatin-treated rat, but neither fraction is present in urine incubated with cisplatin and neither is formed by the interaction of cisplatin or the urinary LMW Pt-containing fractions with kidney cytosol in vitro. It is suggested that the smaller fraction may be derived from the filtration and reabsorption of plasma protein-bound Pt, whereas the larger fraction is more likely to be formed exclusively within the kidney cell.
通过凝胶过滤和离子交换色谱法研究了向成年雄性Wistar大鼠施用顺二氯二氨铂II（顺铂）后铂在尿液和肾脏中的分布情况。在施用顺铂后15分钟内，肾脏细胞溶质中存在几种低分子量（LMW）含铂组分，其色谱性质与用顺铂孵育的尿液中发现的相同。这些组分中铂的浓度迅速下降，4小时后，肾脏细胞溶质中的大部分铂存在于一个高分子量（HMW）组分中，该组分包含2个亚组分。较小的组分（分子量=20000），而非较大的组分（分子量大于250000），也存在于经顺铂处理的大鼠尿液中，但在用顺铂孵育的尿液中均不存在这两种组分，且它们都不是由顺铂或尿液中含低分子量铂的组分与肾脏细胞溶质在体外相互作用形成的。提示较小的组分可能来源于血浆蛋白结合铂的滤过和重吸收，而较大的组分更可能仅在肾细胞内形成。
