鉴定 PtoCP1 启动子中的一个 ，以有效地控制 中的组成型基因表达。
Identifying a in PtoCP1 promoter for efficiently controlling constitutive gene expression in .
机构信息
State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Co, Beijing Forestry University, Beijing, China.
出版信息
PeerJ. 2024 Oct 22;12:e18292. doi: 10.7717/peerj.18292. eCollection 2024.
Gene expression is regulated by transcription factors binding to in promoters. However, efficient for genetic engineering are rarely reported. In this study, we identified an 11 bp in the PtoCP1 promoter that drives strong constitutive gene expression in . A 2,270 bp promoter region upstream of the gene's translation start site was cloned and named ProPtoCP1. This promoter controls GUS reporter gene expression in the roots, leaves, and stems of seedlings. Based on the location and density of , the PtoCP1 promoter was divided into four fragments by 5'-end deletions. GUS staining and RT-qPCR revealed a key at -466 to -441 bp essential for gene expression. Further analysis showed that the MYB-TGACG is a positive regulator, whereas neither MYB nor TGACG alone drove gene expression. This study enhances our understanding of gene expression regulation by and provides a valuable tool for genetic engineering.
基因表达受转录因子结合到启动子中的调控。然而，很少有报道高效的遗传工程。在这项研究中，我们在 PtoCP1 启动子中鉴定出一个 11bp 的 ，它在 中驱动强烈的组成型基因表达。克隆了位于 基因翻译起始位点上游的 2270bp 启动子区域，并将其命名为 ProPtoCP1。该启动子控制 幼苗根、叶和茎中 GUS 报告基因的表达。根据 的位置和密度，通过 5'端缺失将 PtoCP1 启动子分为四个片段。GUS 染色和 RT-qPCR 显示-466 到-441bp 处的关键 对基因表达至关重要。进一步的分析表明，MYB-TGACG 是一个正调控因子，而 MYB 和 TGACG 单独都不能驱动基因表达。本研究增强了我们对 调控基因表达的理解，并为遗传工程提供了有价值的工具。
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