Department of Virology, Veterinary Faculty, Necmettin Erbakan University, Ereğli, 42310 Konya, Turkey.
Department of Parasitology, Veterinary Faculty, Hatay Mustafa Kemal University, Antakya, 31060 Hatay, Turkey.
Bull Entomol Res. 2024 Oct;114(5):631-641. doi: 10.1017/S000748532400052X. Epub 2024 Oct 28.
Deformed wing virus (DWV), which is an important honey bee virus transmitted by , causes colony losses in honey bee colonies. This study aimed to investigate the prevalence and genetic diversity of DWV in honey bees in Türkiye and to determine the role of in the transmission of the genetic variants of DWV. Honey bee samples were collected from 62 apiaries, by simple random sampling, during March 2022 and April 2023. The presence of in collected bee samples was examined using a stereo microscope. Real-time RT-PCR was used for the detection of DWV-A and DWV-B ( virus-1 (VDV-1)) viruses. Genetic characterisation of the positive samples was conducted by sequencing polyprotein genomic region. Considering the infestation rate of 3% as relevant, out of the 62 apiaries examined, 17 (27.4%) were positive. However, DWV-A and VDV-1 specific RNA was not detected in samples. VDV-1 specific RNA was detected in 6.5% (4/62) of the apiaries, whereas DWV-A was not detected in the sampled apiaries. Phylogenetic analysis showed that isolates detected in this study were located in a separate cluster from previously characterised DWV-A and VDV-1 isolates. According to RDP4 and GARD analyses, DWV-VDV-1 recombination breakpoints were detected in field isolates. To the best our knowledge, this is the first report of the presence of VDV-1-DWV recombinants in Türkiye. Further studies are needed to determine the impact of VDV-1-DWV recombinants and their virological and antigenic properties.
变形翅膀病毒（DWV）是一种重要的蜜蜂病毒，通过 传播，导致蜜蜂群的损失。本研究旨在调查土耳其蜜蜂中 DWV 的流行率和遗传多样性，并确定 在 DWV 遗传变异的传播中的作用。2022 年 3 月至 2023 年 4 月，通过简单随机抽样，从 62 个养蜂场采集蜜蜂样本。使用立体显微镜检查采集的蜜蜂样本中 是否存在 。使用实时 RT-PCR 检测 DWV-A 和 DWV-B（病毒-1（VDV-1））病毒。通过测序多蛋白基因组区域对阳性样本进行遗传特征分析。考虑到 3%的 感染率是相关的，在检查的 62 个养蜂场中，有 17 个（27.4%）是阳性的。然而， 在 样本中未检测到 DWV-A 和 VDV-1 特异性 RNA。在 6.5%（4/62）的养蜂场中检测到 VDV-1 特异性 RNA，而在采样的养蜂场中未检测到 DWV-A。系统发育分析表明，本研究中检测到的分离株与先前表征的 DWV-A 和 VDV-1 分离株位于不同的聚类中。根据 RDP4 和 GARD 分析，在田间分离株中检测到 DWV-VDV-1 重组断点。据我们所知，这是土耳其首次报道存在 VDV-1-DWV 重组体。需要进一步研究来确定 VDV-1-DWV 重组体及其病毒学和抗原特性的影响。
