Mo Yuqian, Huang Enyu, Deng Chao, Huang Haofeng, Zhu Ying, Wei Xinlong, Zhong Jinlin, Wang Yuzhi, Huang Zhigang, Zhang Jingjing
Department of Epidemiology and Health Statistics, School of Public Health, Guangdong Medical University, Dongguan, China.
Affiliated Hospital of Guangdong Medical University & Zhanjiang Key Laboratory of Zebrafish Model for Development and Disease, Guangdong Medical University, Zhanjiang, China.
J Cell Physiol. 2025 Jan;240(1):e31474. doi: 10.1002/jcp.31474. Epub 2024 Oct 28.
Gastric cancer (GC) presents a significant global health burden, with metastasis being the leading cause of treatment failure and mortality. NAT10, a regulatory protein involved in mRNA acetylation, has been implicated in various cancers. However, its role in GC, especially concerning metastasis and immune interactions, remains unclear. Utilizing multi-omics data from gastric cancer samples, we conducted comprehensive analyses to investigate NAT10 expression, its correlation with clinical parameters and immune relevance. Bioinformatics analysis and digital image processing were employed for this purpose. Furthermore, in vitro and in vivo experiments were conducted to elucidate the functional role of NAT10 in gastric cancer progression, aiming to provide deeper biological insights. Our findings reveal a significant association between NAT10 expression and various aspects of transcriptional, protein, as well as tumor immunity in GC patients. Additionally, we demonstrated that NAT10 promotes gastric cancer cell proliferation and migration, both in cellular models and in animal studies, suggesting its involvement in early tumor microvascular metastasis. NAT10 emerges as a promising molecular target, offering potential avenues for further research into molecular mechanisms and therapeutic strategies for GC.
胃癌（GC）是一项重大的全球健康负担，转移是治疗失败和死亡的主要原因。NAT10是一种参与mRNA乙酰化的调节蛋白，已被证明与多种癌症有关。然而，其在胃癌中的作用，特别是与转移和免疫相互作用有关的作用，仍不清楚。利用胃癌样本的多组学数据，我们进行了全面分析，以研究NAT10的表达、其与临床参数的相关性以及免疫相关性。为此采用了生物信息学分析和数字图像处理。此外，还进行了体外和体内实验，以阐明NAT10在胃癌进展中的功能作用，旨在提供更深入的生物学见解。我们的研究结果揭示了NAT10表达与GC患者转录、蛋白质以及肿瘤免疫的各个方面之间存在显著关联。此外，我们证明，在细胞模型和动物研究中，NAT10均促进胃癌细胞增殖和迁移，表明其参与早期肿瘤微血管转移。NAT10成为一个有前景的分子靶点，为进一步研究GC的分子机制和治疗策略提供了潜在途径。
