College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China; Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.
Department of Nutrition and Health, China Agricultural University, Beijing 100083, China; Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Government, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Food Chem. 2025 Feb 1;464(Pt 2):141766. doi: 10.1016/j.foodchem.2024.141766. Epub 2024 Oct 23.
This study provides insights into the composition and in-situ structures of the milk fat globule membrane (MFGM) in buffalo milk with different fat globule sizes (0.55 μm and 8.04 μm). Small fat globules contained a higher amount of MFGM proteins and polar lipids, while the content of MFGM components (except for ADPH) was greater per unit membrane area in large fat globules. A total of 386 differentially expressed proteins were identified, with many of the most differentially expressed proteins being associated with human diseases, such as cancer and elderly diseases. The coverage of sphingomyelin and certain MFGM proteins (BTN and PAS 6/7) on large fat globules was higher, potentially linked to the presence of larger, irregular lipid rafts and patch-shaped glycocalyxes, respectively. These findings suggest that small fat globules are suitable for nutritional fortification of buffalo milk, while large fat globules are appropriate for the preparation of functional dairy-based ingredients.
本研究深入了解了不同脂肪球大小（0.55μm 和 8.04μm）的水牛乳中乳脂肪球膜（MFGM）的组成和原位结构。小脂肪球含有更高量的 MFGM 蛋白和极性脂，而大脂肪球中每单位膜面积的 MFGM 成分（除 ADPH 外）含量更高。共鉴定出 386 种差异表达蛋白，其中许多最差异表达蛋白与人类疾病（如癌症和老年疾病）相关。大脂肪球上的鞘磷脂和某些 MFGM 蛋白（BTN 和 PAS 6/7）的覆盖率更高，这可能分别与较大的不规则脂筏和斑块状糖萼的存在有关。这些发现表明，小脂肪球适合作为水牛乳的营养强化剂，而大脂肪球则适合制备功能性乳制品成分。
