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大鼠连络核向前庭小脑的间接通路参与垂直凝视保持。

An Indirect Pathway from the Rat Interstitial Nucleus of Cajal to the Vestibulocerebellum Involved in Vertical Gaze Holding.

机构信息

Department of Neurophysiology, Nara Medical University, Kashihara 634-8521, Japan.

Division of Visual Information Processing, National Institute for Physiological Sciences and School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Okazaki 444-8585, Japan.

出版信息

eNeuro. 2024 Nov 6;11(11). doi: 10.1523/ENEURO.0294-24.2024. Print 2024 Nov.

DOI:10.1523/ENEURO.0294-24.2024
PMID:39467649
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11540594/
Abstract

The neural network, including the interstitial nucleus of Cajal (INC), functions as an oculomotor neural integrator involved in the control of vertical gaze holding. Impairment of the vestibulocerebellum (VC), including the flocculus (FL), has been shown to affect vertical gaze holding, indicating that the INC cooperates with the VC in controlling this function. However, a network between the INC and VC has not been identified. In this study, we aimed to obtain anatomical evidence of a neural pathway from the INC to the VC (the INC-VC pathway) in rats. Injection of dextran-conjugated Alexa Fluor 488 or adeno-associated virus 2-retro (AAV2retro) expressing GFP into the FL or another VC region (uvula/nodulus) did not reveal any retrogradely labeled neurons in the INC, suggesting that INC neurons do not project directly to the VC. Rabies virus-based transsynaptic tracing experiments revealed that the INC-VC pathway is mediated via synaptic connections with the prepositus hypoglossi nucleus (PHN) and medial vestibular nucleus (MVN). The INC neurons in the INC-VC pathway were mainly localized bilaterally within the rostral region of the INC. Transsynaptic tracing experiments involving the INC-FL pathway revealed that INC neurons connected to the FL via the bilateral PHN and MVN. These results indicate that the INC-VC pathway is not a direct pathway but is mediated via the PHN and MVN. These findings can provide clues for understanding the network mechanisms responsible for vertical gaze holding.

摘要

神经网络包括 Cajal 间质核（INC），作为参与垂直凝视保持的眼球运动神经整合器发挥作用。已经表明，前庭小脑（VC）的损伤，包括绒球（FL），会影响垂直凝视保持，这表明 INC 与 VC 合作控制此功能。然而，INC 和 VC 之间的网络尚未确定。在这项研究中，我们旨在获得大鼠 INC 到 VC（INC-VC 通路）的神经通路的解剖学证据。将葡聚糖缀合的 Alexa Fluor 488 或表达 GFP 的腺相关病毒 2- retro（AAV2retro）注入 FL 或另一个 VC 区域（uvula/nodulus），不会在 INC 中发现任何逆行标记神经元，表明 INC 神经元不会直接投射到 VC。基于狂犬病病毒的转导追踪实验表明，INC-VC 通路是通过与前庭下核（PHN）和内侧前庭核（MVN）的突触连接介导的。INC-VC 通路中的 INC 神经元主要位于 INC 的前侧区域的双侧。涉及 INC-FL 通路的转导追踪实验表明，INC 神经元通过双侧 PHN 和 MVN 与 FL 相连。这些结果表明，INC-VC 通路不是直接通路，而是通过 PHN 和 MVN 介导的。这些发现可以为理解负责垂直凝视保持的网络机制提供线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/ec8f14c98481/eneuro-11-ENEURO.0294-24.2024-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/6e0773b72edb/eneuro-11-ENEURO.0294-24.2024-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/64b23931a0da/eneuro-11-ENEURO.0294-24.2024-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/1ee6dda56445/eneuro-11-ENEURO.0294-24.2024-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/d20127c3f499/eneuro-11-ENEURO.0294-24.2024-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/361d2980b759/eneuro-11-ENEURO.0294-24.2024-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/d3f584965c6a/eneuro-11-ENEURO.0294-24.2024-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/ec8f14c98481/eneuro-11-ENEURO.0294-24.2024-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/6e0773b72edb/eneuro-11-ENEURO.0294-24.2024-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/64b23931a0da/eneuro-11-ENEURO.0294-24.2024-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/1ee6dda56445/eneuro-11-ENEURO.0294-24.2024-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/d20127c3f499/eneuro-11-ENEURO.0294-24.2024-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/361d2980b759/eneuro-11-ENEURO.0294-24.2024-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/d3f584965c6a/eneuro-11-ENEURO.0294-24.2024-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/daa8/11540594/ec8f14c98481/eneuro-11-ENEURO.0294-24.2024-g007.jpg

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3
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4
Heterogeneous vestibulocerebellar mossy fiber projections revealed by single axon reconstruction in the mouse.在小鼠中通过单轴突重建揭示的异质性前庭小脑苔藓纤维投射。
J Comp Neurol. 2020 Jul;528(10):1775-1802. doi: 10.1002/cne.24853. Epub 2020 Jan 21.
5
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6
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7
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