Department of PG Studies and Research in Chemistry, Kuvempu University, Shivamogga, Karnataka, India.
Department of Studies in Chemistry, Davangere University, Davangere, Karnataka, India.
Luminescence. 2024 Oct;39(10):e70003. doi: 10.1002/bio.70003.
The fluorescent materials have sparked a lot of research interests due to their unique electronic, optical and chemical characteristics. Here, we are intended to present a simple and facile synthesis of novel orange emitting thiazole-pyridone fluorescent tag (TPFT) by a simple diazo coupling reaction and the structural elucidation was carried out by IR, NMR (H and C), UV-Vis, photoluminescence and HR-MS spectrometry. The solvatochromic behaviour of the TPFT offered crucial information about the formation of hydrazone and azo tautomeric forms. The DFT simulations are computed to calculate HOMO-LUMO energy gap (3.028 eV) of TPFT along with MEP and RDG analyses. Comprehensive LFP visualization is revealed under both normal and UV light conditions (365 nm). The cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to analyse the electrochemical behaviour of the TPFT-modified glassy carbon electrode (MGCE) and exhibited a lower detection limit of 7.89 × 10 M (S/N = 3) with a linear range of 0.5-8.0 μM for DA detection. The live-cell imaging study of TPFT showed a strong blue emission at 453 nm, which generally indicates the existence of fluorescence stability.
荧光材料因其独特的电子、光学和化学特性而引起了广泛的研究兴趣。在这里，我们旨在通过简单的重氮偶联反应来合成新型橙色发射噻唑-吡啶酮荧光标记物（TPFT），并通过红外光谱（IR）、核磁共振（H 和 C）、紫外可见光谱、光致发光和高分辨质谱（HR-MS）光谱对其结构进行了阐明。TPFT 的溶剂化行为提供了关于腙和偶氮互变异构形式形成的关键信息。通过密度泛函理论（DFT）模拟计算了 TPFT 的 HOMO-LUMO 能隙（3.028 eV），同时进行了 MEP 和 RDG 分析。在正常和紫外光条件下（365nm）均可以全面观察到 LFP。循环伏安法（CV）和差分脉冲伏安法（DPV）用于分析 TPFT 修饰的玻碳电极（MGCE）的电化学行为，DA 的检测下限为 7.89×10-7 M（S/N=3），线性范围为 0.5-8.0 μM。TPFT 的活细胞成像研究显示在 453nm 处有强的蓝色发射，这通常表明荧光稳定性的存在。
