Ma Xiaoyun, Feng Mengnan, Wang Qiang, Li Yu, Cao Dongmei
College of Horticulture, Shanxi Agricultural University, Taiyuan 030031, Shanxi, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Oct 25;40(10):3629-3648. doi: 10.13345/j.cjb.240074.
To explore the role and metabolic differences of plant hormones in regulating the flower bud size of daylily, we collected the flower buds from two daylily varieties 'Datong Huanghua' and 'Dongbei Huanghua' at the young bud, green, yellowing, and yellow stages for transcriptome sequencing. The differentially expressed genes (DEGs) were screened, and the exogenous plant hormone spraying experiments were conducted. A total of 199 DEGs related to the biosynthesis and metabolism of plant hormones was screened out at different flower development stages of 'Datong Huanghua' and 'Dongbei Huanghua'. These genes regulated the biosynthesis and metabolism of six plant hormones: abscisic acid, gibberellin, auxin, jasmonate, cytokinin, and ethylene. The DEGs associated with auxin were the most, which suggested that auxin played a role in regulating flower bud development. The auxin response factor () presented up-regulated expression at all the four stages of flower bud development, indicating that played a positive regulatory role throughout the flower bud development of daylily. The experiments with exogenous spraying of six hormones further verified that indole-3-acetic acid (IAA) significantly promoted the growth and increased the nutrient content in the flower buds of 'Datong Huanghua', suggesting that IAA played a role in regulating flower bud development. Our results laid a theoretical foundation for probing into the regulatory mechanism of flower bud development of 'Datong Huanghua' and 'Dongbei Huanghua'.
为探究植物激素在调控黄花菜花芽大小中的作用及代谢差异，我们采集了黄花菜品种‘大同黄花’和‘东北黄花’在幼蕾期、绿色期、泛黄期和黄色期的花蕾进行转录组测序。筛选出差异表达基因（DEGs），并进行了外源植物激素喷施实验。在‘大同黄花’和‘东北黄花’的不同花发育阶段共筛选出199个与植物激素生物合成和代谢相关的DEGs。这些基因调控脱落酸、赤霉素、生长素、茉莉酸、细胞分裂素和乙烯六种植物激素的生物合成和代谢。与生长素相关的DEGs最多，这表明生长素在调控花芽发育中起作用。生长素响应因子（）在花芽发育的四个阶段均呈上调表达，表明其在黄花菜整个花芽发育过程中起正向调控作用。六种激素的外源喷施实验进一步证实，吲哚 - 3 - 乙酸（IAA）显著促进了‘大同黄花’花蕾的生长并增加了其营养含量，表明IAA在调控花芽发育中起作用。我们的研究结果为探究‘大同黄花’和‘东北黄花’花芽发育的调控机制奠定了理论基础。
