未经辐射暴露的抗辐射细菌的分离
Isolation of radiation-resistant bacteria without exposure to irradiation.
作者信息
Sanders S W, Maxcy R B
出版信息
Appl Environ Microbiol. 1979 Sep;38(3):436-9. doi: 10.1128/aem.38.3.436-439.1979.
Abstract
Resistance to desiccation was utilized in the selection of highly radiation-resistant asporogenous bacteria from non-irradiated sources. A bacterial suspension in phosphate buffer was dried in a thin film at 25 degrees C and 33% relative humidity. Storage under these conditions for 15 days or more reduced the number of radiation-sensitive bacteria. Further selection for radiation-resistant bacteria was obtained by irradiation of bacteria on velveteen in the replication process, thereby avoiding the toxic effect of irradiated media. The similarity of radiation resistance and identifying characteristics in irradiated and non-irradiated isolates should allay some concerns that highly radiation-resistant bacteria have been permanently altered by radiation selection.
摘要
利用对干燥的抗性从未辐照的来源中筛选高度耐辐射的无芽孢细菌。将磷酸盐缓冲液中的细菌悬浮液在25℃和33%相对湿度下干燥成薄膜。在这些条件下储存15天或更长时间可减少辐射敏感细菌的数量。通过在复制过程中对天鹅绒布上的细菌进行辐照，进一步筛选耐辐射细菌，从而避免辐照培养基的毒性作用。辐照和未辐照分离株在辐射抗性和鉴定特征方面的相似性应能减轻一些关于高度耐辐射细菌已因辐射选择而发生永久性改变的担忧。
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