Pereira Júnior Jucimauro de Araújo, Costa Domickson Silva, Silva Arthur Dos Santos da, Santos Gracienhe Gomes Dos, Santos Arthur Felipe Lima Dos, Silva Aldeize Driely Cardoso da, Couto Márcia Valéria Silva do, Cordeiro Carlos Alberto Martins, Martins Maurício Laterça, Sousa Natalino da Costa
Aquaculture Department, AQUOS: Aquatic Organisms Health Laboratory, UFSC, Florianópolis, Santa Catarina, Brazil.
Institute of Coastal Studies, Federal University of Pará, UFPA, Bragança, Pará, Brazil.
J Fish Dis. 2025 Jan;48(1):e14039. doi: 10.1111/jfd.14039. Epub 2024 Oct 29.
The objective of this study was to evaluate the effect of dietary supplementation with Citrus sinensis (L.) Osbeck essential oil on the growth, immune system, and resistance to Aeromonas hydrophila infection in Colossoma macropomum fingerlings. The experiment was conducted with five treatments (control diet, Tween80 diet, and diets supplemented with 200, 400, and 800 mg L of C. sinensis essential oil) with four replicates. At the end of the experimental period, growth parameters were measured, and blood samples were collected for thrombogram, leukogram, and phagocytic activity analysis. A bacterial challenge with A. hydrophila was conducted for 96 h. C. macropomum fingerlings that were fed with 400 and 800 mg L of C. sinensis essential oil had the highest growth parameters, with final weights of 533.18 ± 2.03 mg and 531.91 ± 2.67 mg, respectively, and an increase in the number of thrombocytes, lymphocytes, monocytes, and neutrophils, as well as higher phagocytosis rates compared to the control group. Regarding the challenge, fish in the 400 and 800 mg L treatments also exhibited the lowest cumulative mortality rate (26.66% ± 3.33%). Therefore, supplementation with C. sinensis essential oil promotes growth, improves health, and enhances resistance to A. hydrophila in C. macropomum fingerlings.
本研究的目的是评估日粮中添加脐橙（Citrus sinensis (L.) Osbeck）精油对大口胭脂鱼（Colossoma macropomum）幼鱼生长、免疫系统及嗜水气单胞菌（Aeromonas hydrophila）感染抵抗力的影响。实验设置了五个处理组（对照日粮、吐温80日粮以及添加200、400和800 mg/L脐橙精油的日粮），每组四个重复。在实验期结束时，测量生长参数，并采集血样进行血栓图、白细胞图和吞噬活性分析。用嗜水气单胞菌进行96小时的攻毒实验。投喂400和800 mg/L脐橙精油的大口胭脂鱼幼鱼生长参数最高，终末体重分别为533.18±2.03 mg和531.91±2.67 mg，与对照组相比，其血小板、淋巴细胞、单核细胞和中性粒细胞数量增加，吞噬率更高。在攻毒实验方面，400和800 mg/L处理组的鱼累积死亡率也最低（26.66%±3.33%）。因此，添加脐橙精油可促进大口胭脂鱼幼鱼生长、改善健康状况并增强其对嗜水气单胞菌的抵抗力。
