Household and Structural Urban Entomology Laboratory, Vector Control Research Unit, School of Biological Science, Universiti Sains Malaysia, Minden, Penang 11800, Malaysia.
J Insect Sci. 2024 Sep 1;24(5). doi: 10.1093/jisesa/ieae096.
The American cockroach, Periplaneta americana (Blattodea: Blattidae), is a prevalent urban pest with significant public health implications. This study aimed to develop and validate novel microsatellite markers to understand the genetic diversity and population genetic structure of P. americana. In this study, a total of 397,898 microsatellite markers were developed based on 24.6 million genomic DNA sequences. Twenty microsatellite markers were selected and amplified with varying numbers of alleles ranging from 0 to 35. Seven out of 20 markers were characterized for their polymorphism and amplification efficiency. The polymorphic information content (PIC) values of these markers were high (0.669 to 0.950) implying their effectiveness. These markers also revealed 7 to 35 alleles per locus across tested samples, highlighting their utility in assessing the extensive genetic variation within Periplaneta americana populations. These results provide insightful information that may be applied to the genetic analysis of the American cockroach population using the developed species-specific microsatellite marker.
美洲大蠊（Periplaneta americana）（蜚蠊目：蜚蠊科）是一种普遍存在的城市害虫，对公共卫生有重大影响。本研究旨在开发和验证新的微卫星标记，以了解美洲大蠊的遗传多样性和种群遗传结构。在本研究中，基于 2460 万条基因组 DNA 序列，共开发了 397898 个微卫星标记。选择了 20 个微卫星标记，其等位基因数从 0 到 35 不等。对其中的 7 个标记进行了多态性和扩增效率的特征分析。这些标记的多态信息含量（PIC）值较高（0.669 至 0.950），表明其有效性。这些标记在测试样本中每一位点显示 7 到 35 个等位基因，突出了它们在评估美洲大蠊种群广泛遗传变异方面的效用。这些结果提供了有价值的信息，可应用于使用开发的种特异性微卫星标记对美洲大蠊种群进行遗传分析。
