Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Departamento de Ingeniería Química y Ambiental, Facultad de Ingeniería, Universidad Nacional de Colombia, C.P. 111321, Bogotá, Colombia.
Int J Syst Evol Microbiol. 2024 Oct;74(10). doi: 10.1099/ijsem.0.006565.
Four yeast isolates obtained from tree bark and fermenting sap of spp. and insects in Colombia and Japan were phylogenetically related to based on analyses of the sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene. The novel species differs from by 20 nt substitutions and 5 indels in the D1/D2 sequences. A phylogenomic analysis suggested that these species are related to , the genus and a small clade containing , and . Our genomic analyses suggest that the novel species and should be separated in a novel yeast genus. We propose the genus gen. nov. to accommodate these species and the species f.a., sp. nov. (CBS 18021; MycoBank MB856172) to accommodate the Colombian and Japanese isolates. The Colombian isolate of differs from the Japanese isolates by three nt substitutions and one indel and two substitutions and one indel in the ITS and D1/D2 sequences, respectively, showing that they were conspecific. We also propose the new species sp. nov. to validate this species according to the rules of the International Code of Nomenclature for algae, fungi and plants.
从哥伦比亚和日本的 和昆虫的树皮和发酵汁液中分离得到的四个酵母分离株，基于对内转录间隔区（ITS）区域和大亚基 rRNA 基因的 D1/D2 结构域序列的分析，与 具有系统发育关系。新种与 在 D1/D2 序列中存在 20 个核苷酸取代和 5 个插入/缺失。系统基因组分析表明，这些物种与 、属 和包含 、 和 的小分支有关。我们的基因组分析表明，新型物种和 应在一个新的酵母属中分离。我们提出了新属 来容纳这些物种和种 f.a.，sp. nov.（CBS 18021；MycoBank MB856172），以容纳哥伦比亚和日本的分离株。与日本分离株相比，哥伦比亚分离株在 ITS 和 D1/D2 序列中分别有三个核苷酸取代和一个插入/缺失，以及两个取代和一个插入/缺失，表明它们是同一种。我们还根据藻类、真菌和植物国际命名法规的规则，提出了新的物种 sp. nov. 来验证这个物种。
