Varyani Leila, Ahmadpanah Niloofar, Kasiri Rozhin, Shahzamani Shadman, Tomraee Simindokht, Jafari Aref, Mirjalili Hosna, Asl Nassim Seyedi
School of Medicine, Babol University of Medical Sciences, Babol, Iran.
Biotechnology, Science Department, Faculty of Science, Engineering and Environment, University of Salford, Greater Manchester, United Kingdom.
Tissue Cell. 2024 Dec;91:102590. doi: 10.1016/j.tice.2024.102590. Epub 2024 Oct 21.
Diabetic wound is one of the most common and costly complication in diabetic patients. Hence, numerous studies have been carried out to discover a suitable approach to enhance the process of wound healing. Biological hydrogels are commonly utilized for wound healing due to their suitable properties among different materials available. Herein we investigated whether human amniotic membrane hydrogel (hAMH) loaded with human placental mesenchymal stem cells (PlaMSCs)-derived exosomes could promote healing in diabetic rats. Sixty diabetic rats were randomly assigned into the control group, hAMH group, exosome group, and hAMH+Exosome group. According to the phases of wound healing, sampling was done on days 7, 14, and 21 for further assessments. Our findings showed a significant increase in wound contraction rate, new epidermal length, fibroblast and blood vessel count, collagen density, and the levels of antioxidative factors (GSH, SOD, and CAT) in the treatment groups compared to the control group, with more pronounced effects observed in the hAMH+Exosome group. Furthermore, the levels of bFGF and VEGF gene expression significantly increased in each treatment group when compared to the control group, with the highest levels observed in the hAMH+Exosome group. This occurred as the hAMH+Exosome group showed a greater decrease in neutrophil count, the expression of TNF-α and IL-1β genes, and the levels of an oxidative factor (MDA) compared to the other groups. In summary, the combination of hAMH and PlaMSCs-derived exosomes was determined to have a more significant effect on healing diabetic wounds.
糖尿病伤口是糖尿病患者中最常见且代价高昂的并发症之一。因此，人们开展了大量研究以探寻促进伤口愈合的合适方法。由于生物水凝胶在现有不同材料中具有适宜特性，故而常用于伤口愈合。在此，我们研究了负载人胎盘间充质干细胞（PlaMSCs）衍生外泌体的人羊膜水凝胶（hAMH）是否能促进糖尿病大鼠的伤口愈合。60只糖尿病大鼠被随机分为对照组、hAMH组、外泌体组和hAMH + 外泌体组。根据伤口愈合阶段，在第7天、14天和21天进行取样以作进一步评估。我们的研究结果显示，与对照组相比，各治疗组的伤口收缩率、新表皮长度、成纤维细胞和血管数量、胶原蛋白密度以及抗氧化因子（谷胱甘肽、超氧化物歧化酶和过氧化氢酶）水平均显著增加，其中hAMH + 外泌体组的效果更为显著。此外，与对照组相比，各治疗组的碱性成纤维细胞生长因子（bFGF）和血管内皮生长因子（VEGF）基因表达水平均显著升高，hAMH + 外泌体组的水平最高。这是因为与其他组相比，hAMH + 外泌体组的中性粒细胞计数、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）基因表达以及氧化因子（丙二醛，MDA）水平下降更为明显。总之，hAMH与PlaMSCs衍生外泌体的组合对糖尿病伤口愈合具有更显著的效果。
