Van den Abbeele P, Heine R G, Van de Vliet M, Favre L, Tytgat H L P, Sprenger N, Deyaert S, Baudot A, Nutten S
Cryptobiotix SA, 9052 Ghent, Belgium.
Nestlé Health Science, 1800 Vevey, Switzerland.
Benef Microbes. 2024 Oct 25;16(2):171-185. doi: 10.1163/18762891-bja00048.
Cow's milk protein allergy (CMPA) in infancy is associated with intestinal microbial dysbiosis, characterised by low Bifidobacteriaceae levels. The present study aimed to investigate the impact of two human milk oligosaccharides (HMO), lactose (L), and their combination on the faecal microbiome and metabolome of infants with CMPA. Stool samples of 12 term infants with probable CMPA (mean age 4.3 months) were analysed using a validated intestinal fermentation assay (SIFR® technology). For each substrate (i.e. HMO (2'-fucosyllactose [2'-FL] and lacto-N-neotetraose [LNnT]), L and HMO + L), taxonomic microbiome characterisation and untargeted metabolite profiling were performed at multiple timepoints. At baseline, the tested faecal microbiota overall displayed low abundances of Bifidobacteriaceae. Fermentation with either HMO or lactose significantly enriched Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium pseudocatenulatum and, for HMO + L, also Bifidobacterium bifidum. The increase in HMO-utilising bifidobacteria was associated with a significant rise in levels of short-chain fatty acids, aromatic lactic acids and N-acetylated amino acids, with additive effects being observed for HMO + L. The above data suggest that the combination of 2'-FL, LNnT and lactose helps to alleviate the previously reported CMPA-associated intestinal bacterial dysbiosis and induces the production of several beneficial metabolites. The clinical significance of these findings for infants with CMPA requires further investigation.
婴儿期牛奶蛋白过敏（CMPA）与肠道微生物群失调有关，其特征是双歧杆菌科水平较低。本研究旨在调查两种人乳寡糖（HMO）、乳糖（L）及其组合对CMPA婴儿粪便微生物组和代谢组的影响。使用经过验证的肠道发酵试验（SIFR®技术）分析了12名足月疑似CMPA婴儿（平均年龄4.3个月）的粪便样本。对于每种底物（即HMO（2'-岩藻糖基乳糖[2'-FL]和乳糖-N-新四糖[LNnT]）、L和HMO+L），在多个时间点进行了微生物分类学特征分析和非靶向代谢物谱分析。在基线时，测试的粪便微生物群总体上显示双歧杆菌科丰度较低。用HMO或乳糖发酵可显著富集短双歧杆菌、长双歧杆菌、假链状双歧杆菌，对于HMO+L，还可富集两歧双歧杆菌。利用HMO的双歧杆菌增加与短链脂肪酸、芳香族乳酸和N-乙酰化氨基酸水平的显著升高有关，HMO+L观察到相加效应。上述数据表明，2'-FL、LNnT和乳糖的组合有助于缓解先前报道的与CMPA相关的肠道细菌失调，并诱导产生几种有益代谢物。这些发现对CMPA婴儿的临床意义需要进一步研究。
