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三碘甲状腺原氨酸(T3)ELISA 检测试剂盒在鼠粪样本中的验证。

Validation of a triiodothyronine (T3) ELISA for mouse fecal samples.

机构信息

Biological Sciences, Southern Illinois University Edwardsville, Edwardsville, Illinois, USA.

Biomedical and Craniofacial Sciences, Southern Illinois University School of Dental Medicine, Alton, Illinois, USA.

出版信息

Physiol Rep. 2024 Nov;12(21):e70115. doi: 10.14814/phy2.70115.

Abstract

Acquiring sufficient blood for hormone analysis in mice can be a limiting step. Hormone analysis techniques using non-invasive sample collection have been vigorously developed for endangered species, from whom blood sampling is prohibited, or from species that are otherwise difficult to handle in a laboratory setting. Because there are interactions between glucocorticoids and thyroid hormones (T3 and T4), reducing the animal's "distress" during sample collection is imperative. Measurement of fecal T3 provides less sensitive, baseline information regarding thyroid function while permitting a non-invasive technique for more frequent sampling. We demonstrated that using a methanol extraction protocol produced the most reliable fecal T3 measurement in an enzyme-linked immunosorbent assay (ELISA). We found that during a thyroid hormone-treated state, fecal and plasma T3 measurements from mice are directly related, while during a methimazole-treated state, fecal and plasma T3 measurements from mice are inversely related. Fecal samples are a useful way to monitor thyroid hormone function in laboratory mice.

摘要

获取用于激素分析的足够血液样本可能是一个限制步骤。对于那些禁止采血的濒危物种,或者对于那些在实验室环境中难以处理的物种,人们已经大力开发了使用非侵入性样本采集的激素分析技术。由于糖皮质激素和甲状腺激素(T3 和 T4)之间存在相互作用,因此在采集样本时必须减轻动物的“痛苦”。粪便 T3 的测量提供了关于甲状腺功能的不那么敏感的基线信息,同时允许更频繁地进行非侵入性采样。我们证明,使用甲醇提取方案在酶联免疫吸附测定(ELISA)中可产生最可靠的粪便 T3 测量值。我们发现,在甲状腺激素处理状态下,来自小鼠的粪便和血浆 T3 测量值直接相关,而在甲巯咪唑处理状态下,来自小鼠的粪便和血浆 T3 测量值呈反比关系。粪便样本是监测实验小鼠甲状腺激素功能的一种有用方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2157/11521789/67dc870170e7/PHY2-12-e70115-g007.jpg

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