休眠存活调节剂和促进复苏因子抗原在区分活动性和潜伏性结核病中的作用：系统评价和荟萃分析。
Role of dormancy survival regulator and resuscitation-promoting factors antigens in differentiating between active and latent tuberculosis: a systematic review and meta-analysis.
机构信息
Clinical Laboratory and Blood Transfusion Department, No. 908th Hospital of Joint Logistic Support Force, No. 1028 Jinggangshan Avenue, Qingyunpu District, Nanchang, Jiangxi, 330002, China.
出版信息
BMC Pulm Med. 2024 Oct 29;24(1):541. doi: 10.1186/s12890-024-03348-4.
BACKGROUND
Dormancy survival regulator (DosR) and resuscitation-promoting factor (Rpf) antigens of Mycobacterium tuberculosis are activated during dormant phase of tuberculosis (TB). This study evaluates the differential immunogenicity potentials of DosR and Rpf antigens in individuals with latent tuberculosis infection (LTBI) and active TB patients.
METHODS
After a literature search in electronic databases, studies were selected by following precise eligibility criteria. Outcomes were synthesized systematically, and meta-analyses were performed to estimate standardized mean differences (SMDs) in interferon-gamma (IFNγ) levels, and IFNγ positive immune cells between individuals with LTBI and active TB patients.
RESULTS
Twenty-six studies (1278 individuals with LTBI and 1189 active TB patients) were included. DosR antigens Rv0569 (Standardized mean difference; SMD 2.44 [95%CI: 1.21, 3.66]; p < 0.0001), Rv1733c (SMD 0.60 [95%CI: 0.14, 1.07]; p = 0.011), Rv1735c (SMD 1.16 [95%CI: 0.44, 1.88]; p = 0.002), Rv1737c (SMD 1.26 [95%CI: 0.59, 1.92]; p < 0.0001), Rv2029c (SMD 0.89 [95%CI: 0.35, 1.42]; p = 0.002), RV2626c (SMD 1.24 [95%CI: 0.45, 2.02); p = 0.002), and Rv2628 (SMD 0.65 [95%CI: 0.38, 0.91]; p < 0.0001) and Rpf antigens Rv0867c (SMD 1.33 [95%CI: 0.48, 2.18]; p = 0.002), Rv1009 (SMD 0.65 [95%CI: 0.05, 1.25]; p = 0.034), and Rv2450c (SMD 1.54 [95%CI: 0.92, 2.16]; p < 0.0001) elicited higher IFNγ levels in individuals with LTBI in comparison with active TB patients. IFNγ-positive immunoresponsive cells were significantly higher in individuals with LTBI than in active TB patients for antigens Rv1733c (SMD 1.02 [95%CI: 0.15, 1.88]; p = 0.021), Rv2029c (SMD 0.57 [95%CI: 0.05, 1.09]; p = 0.031), and Rv2628 [SMD 0.38 [95%CI: 0.15, 0.61]; p = 0.001).
CONCLUSION
DosR antigens Rv0569, Rv1733c, Rv1735c, Rv1737c, RV2626c, Rv2628, and Rv2029c, and Rpf antigens Rv0867c, Rv1009, and Rv2450c are found to elicit immune responses differently in individuals with LTBI and active TB patients.
背景
结核休眠存活调节蛋白（DosR）和复苏促进因子（Rpf）抗原在结核分枝杆菌休眠期被激活。本研究评估了潜伏结核感染（LTBI）个体和活动性结核病患者中 DosR 和 Rpf 抗原的不同免疫原性潜力。
方法
在电子数据库中进行文献检索后，按照严格的纳入标准选择研究。系统地综合结果，并进行荟萃分析以估计干扰素-γ（IFNγ）水平和 LTBI 个体与活动性结核病患者之间 IFNγ阳性免疫细胞的标准化均数差（SMD）。
结果
纳入了 26 项研究（1278 名 LTBI 个体和 1189 名活动性结核病患者）。DosR 抗原 Rv0569（SMD 2.44 [95%CI：1.21，3.66]；p<0.0001）、Rv1733c（SMD 0.60 [95%CI：0.14，1.07]；p=0.011）、Rv1735c（SMD 1.16 [95%CI：0.44，1.88]；p=0.002）、Rv1737c（SMD 1.26 [95%CI：0.59，1.92]；p<0.0001）、Rv2029c（SMD 0.89 [95%CI：0.35，1.42]；p=0.002）、RV2626c（SMD 1.24 [95%CI：0.45，2.02]；p=0.002）和 Rv2628（SMD 0.65 [95%CI：0.38，0.91]；p<0.0001）以及 Rpf 抗原 Rv0867c（SMD 1.33 [95%CI：0.48，2.18]；p=0.002）、Rv1009（SMD 0.65 [95%CI：0.05，1.25]；p=0.034）和 Rv2450c（SMD 1.54 [95%CI：0.92，2.16]；p<0.0001）在 LTBI 个体中引起更高的 IFNγ水平，与活动性结核病患者相比。与活动性结核病患者相比，LTBI 个体的 IFNγ阳性免疫反应细胞显著更高，针对抗原 Rv1733c（SMD 1.02 [95%CI：0.15，1.88]；p=0.021）、Rv2029c（SMD 0.57 [95%CI：0.05，1.09]；p=0.031）和 Rv2628 [SMD 0.38 [95%CI：0.15，0.61]；p=0.001）。
结论
DosR 抗原 Rv0569、Rv1733c、Rv1735c、Rv1737c、RV2626c、Rv2628 和 Rv2029c 以及 Rpf 抗原 Rv0867c、Rv1009 和 Rv2450c 在 LTBI 个体和活动性结核病患者中引起不同的免疫反应。
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