Istituto Nazionale per le Malattie, Infettive "L. Spallanzani", Via Portuense 292, Rome 00149, Italy.
Eur Respir J. 2010 Jul;36(1):135-42. doi: 10.1183/09031936.00140009. Epub 2009 Nov 19.
Interferon-gamma release assays based on region of difference 1 antigens have improved diagnosis of latent tuberculosis infection (LTBI). However, these tests cannot discriminate between recently acquired infection (higher risk of progression to active tuberculosis) and remote LTBI. The objective of the present study was to evaluate the T-cell interferon-gamma responses to Mycobacterium tuberculosis DosR-regulon-encoded antigens (latency antigens) compared with QuantiFERON TB-Gold In-Tube (QFT-GIT) in subjects at different stages of tuberculosis. A total of 16 individuals with remote LTBI and 23 with recent infection were studied; 15 controls unexposed to M. tuberculosis and 50 patients with active tuberculosis and 45 with cured tuberculosis were also analysed. The results indicated that subjects with remote LTBI showed significantly higher whole-blood interferon-gamma responses to M. tuberculosis latency antigen Rv2628 than did individuals with recent infection, active tuberculosis and controls (p<0.003), whereas no significant differences between these groups were found for other latency antigens tested (Rv2626c, Rv2627c, Rv2031c and Rv2032). The proportion of responders to Rv2628 was five-fold higher among QFT-GIT-positive-individuals with remote infection than among those with recently acquired infection. These data suggest that responses to M. tuberculosis latency antigen Rv2628 may associate with immune-mediated protection against tuberculosis. In contact-tracing investigations, these preliminary data may differentiate recent (positive QFT-GIT results without responses to Rv2628) from remote infection (positive to both tests).
基于差异区域 1 抗原的干扰素-γ释放检测已提高了潜伏性结核感染（LTBI）的诊断。然而，这些检测无法区分新近获得的感染（进展为活动性结核病的风险较高）和陈旧性 LTBI。本研究旨在评估结核分枝杆菌 DosR 调控子编码抗原（潜伏抗原）的 T 细胞干扰素-γ反应与 QuantiFERON TB-Gold In-Tube（QFT-GIT）在不同结核病阶段的受试者中的差异。共研究了 16 例陈旧性 LTBI 患者和 23 例近期感染患者，还分析了 15 例未接触过结核分枝杆菌的对照者、50 例活动性结核病患者和 45 例已治愈结核病患者。结果表明，陈旧性 LTBI 患者全血对结核分枝杆菌潜伏抗原 Rv2628 的干扰素-γ反应明显高于近期感染、活动性结核病和对照组（p<0.003），而对其他测试的潜伏抗原（Rv2626c、Rv2627c、Rv2031c 和 Rv2032）未发现这些组之间有显著差异。在 QFT-GIT 阳性的近期感染患者中，对 Rv2628 有反应者的比例是近期获得性感染患者的五倍。这些数据表明，对结核分枝杆菌潜伏抗原 Rv2628 的反应可能与针对结核病的免疫介导保护有关。在接触者追踪调查中，这些初步数据可能区分近期（QFT-GIT 结果阳性但对 Rv2628 无反应）和陈旧性感染（两种检测均阳性）。
