Department of Ultrasound, the Second Affiliated Hospital of Qiqihar Medical College, 161006 Qiqihar, Heilongjiang, China.
Department of Magnetic Resonance Imaging, the Second Affiliated Hospital of Qiqihar Medical College, 161006 Qiqihar, Heilongjiang, China.
Front Biosci (Landmark Ed). 2024 Oct 18;29(10):360. doi: 10.31083/j.fbl2910360.
Identify transcription factors and target genes associated with prostate cancer, offering new therapy approaches.
Gene Set Enrichment Analysis (GSEA) investigates early 2 factor (E2F) transcription factor family roles in prostate cancer using the TCGA database. Survival analysis examined E2F factors and patient survival connections. Dataset analysis identified E2F1-involved key genes. Quantitative Real-time PCR (qPCR), which combines ultrasound-guided methods to collect clinical samples from prostate cancer patients, was utilized to determine the expression levels of and its target genes in patient samples and cancer cells. The effect of and its target gene expression alterations on prostate cell proliferation was examined utilizing the cell counting kit-8 (CCK8) technique. Double fluorescence enzyme experiment verified E2F1-target gene connections.
E2F family genes induce prostate cancer and show correlated co-expression. , , , , and were considerably over-expressed in prostate cancer tissues. While and were notably underexpressed, there was no statistically important change in the expression between prostate cancer and surrounding tissues. High expression of genes is associated with lower patient survival. The transmemrane protein 132 () was identified as a key gene for action and is associated with poor prognosis in patients. The essential gene for function, , was discovered. According to the qPCR results, and are considerably expressed in cancer cells and patient samples. Interfering with its expression significantly inhibited the proliferation ability of cancer cells. The double luciferase experiment showed that regulates the expression level in phase by binding directly to the promoter.
The E2F transcription factor family induces prostate cancer and correlates with poor prognosis. directly regulates by binding its promoter and controlling the degree of protein expression, thereby affecting cancer cell growth.
鉴定与前列腺癌相关的转录因子和靶基因，为前列腺癌的治疗提供新的方法。
使用 TCGA 数据库，通过基因集富集分析（GSEA）来研究早期 2 因子（E2F）转录因子家族在前列腺癌中的作用。生存分析研究了 E2F 因子与患者生存的关系。通过数据分析确定了 E2F1 相关的关键基因。定量实时 PCR（qPCR）结合超声引导方法从前列腺癌患者中收集临床样本，用于确定患者样本和癌细胞中基因及其靶基因的表达水平。利用细胞计数试剂盒-8（CCK8）技术，研究了基因及其靶基因表达改变对前列腺细胞增殖的影响。双荧光酶实验验证了 E2F1 靶基因的关系。
E2F 家族基因诱导前列腺癌，并表现出相关的共表达。E2F1、E2F2、E2F3A、E2F3B 和 E2F4 在前列腺癌组织中表达明显上调。而在前列腺癌和周围组织之间，基因的表达没有显著差异。基因的高表达与患者的低生存率相关。跨膜蛋白 132（）被鉴定为基因作用的关键基因，与患者的不良预后相关。发现了基因功能的必需基因。根据 qPCR 结果，基因在癌细胞和患者样本中表达明显上调。干扰其表达显著抑制了癌细胞的增殖能力。双荧光素酶实验表明，基因通过直接结合基因的启动子来调节基因的表达水平，从而影响癌细胞的生长。
E2F 转录因子家族诱导前列腺癌并与不良预后相关。基因通过直接结合其启动子来调节基因的表达水平，从而控制蛋白表达的程度，影响癌细胞的生长。
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