Tsin A T, Lam K W
Biochem Biophys Res Commun. 1986 Feb 13;134(3):1209-14. doi: 10.1016/0006-291x(86)90379-7.
Retinyl palmitate hydrolase (RPH) activity of bovine tissues was estimated from retinol formation following incubation of tissue homogenates with all-trans retinyl palmitate. The quantity of retinol produced in the incubation mixture was analyzed by high-performance liquid chromatography. RPH activities of retinal pigment epithelium (RPE), liver, retina, muscle and brain were 194.2, 138.0, 72.5, 25.0 and 5.1 units/gm protein respectively. The RPH activity in the retina was far above that attributable solely to RPE contaminations. The presence of RPH in the retina suggests that retina can utilize retinyl esters for the formation of visual pigments and/or cellular metabolism.
通过将组织匀浆与全反式视黄醇棕榈酸酯一起孵育后，根据视黄醇的生成量来估算牛组织中视黄醇棕榈酸酯水解酶（RPH）的活性。孵育混合物中产生的视黄醇量通过高效液相色谱法进行分析。视网膜色素上皮（RPE）、肝脏、视网膜、肌肉和大脑的RPH活性分别为194.2、138.0、72.5、25.0和5.1单位/克蛋白质。视网膜中的RPH活性远高于仅由RPE污染所导致的活性。视网膜中存在RPH表明视网膜可以利用视黄醇酯来形成视觉色素和/或进行细胞代谢。
