Dai Jianan, Zhang Xin
Department of Chemistry, Research Center for Industries of the Future, Westlake University, 600 Dunyu Road, Hangzhou 310030, Zhejiang Province, China.
Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou 310024, Zhejiang Province, China.
Chem Biomed Imaging. 2023 Oct 26;1(9):796-816. doi: 10.1021/cbmi.3c00091. eCollection 2023 Dec 25.
Fluorescence lifetime has significant applications in the field of fluorescence microscopy. Effective modulation of fluorescence lifetime can be achieved by controlling the radiative versus nonradiative processes of fluorophores. In this review, we systematically analyze and summarize chemical approaches that achieve fluorescence lifetime modulation for three different types of fluorophores, including small molecules, quantum dots, and metal complexes. In particular, this review is focused on the chemical mechanisms underlying fluorescence lifetime, the structure-function relationship that defines how chemical regulation is achieved, and the chemical principles that can be used to modulate different scaffolds of fluorophores. We aim to provide important resources for gaining a deeper understanding of fluorescence lifetime modulation, through in-depth investigation into the modulation mechanisms of various fluorescence systems. Perspectives are also proposed to enable future investigation on fluorescence lifetime modulation, a field that bears promises to drive the advancement and application of fluorescence imaging technology.
荧光寿命在荧光显微镜领域有着重要应用。通过控制荧光团的辐射与非辐射过程，可以实现对荧光寿命的有效调制。在本综述中，我们系统地分析和总结了实现三种不同类型荧光团（包括小分子、量子点和金属配合物）荧光寿命调制的化学方法。特别地，本综述聚焦于荧光寿命背后的化学机制、定义如何实现化学调控的结构 - 功能关系，以及可用于调制不同荧光团支架的化学原理。我们旨在通过深入研究各种荧光系统的调制机制，为更深入理解荧光寿命调制提供重要资源。还提出了展望，以推动未来对荧光寿命调制的研究，这一领域有望推动荧光成像技术的进步和应用。
