Chen Xuejie, Liu Wenya, Liao Yangjie, Xiang Xin, Yang Yue, Tian Li, Gao Xiang, Cao Qian, Guo Hong, Chao Kang, Song Fengmei, Liu Rongbei, Li Ying, Ye Shuyu, Wang Jiangang, Yang Fei, Wang Xiaoyan
Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha, 410013, China.
Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Environ Health (Wash). 2024 May 3;2(7):453-464. doi: 10.1021/envhealth.3c00212. eCollection 2024 Jul 19.
Microcystin-LR (MC-LR), the most prevalent and diverse cyanotoxin produced by harmful cyanobacterial blooms, has been linked to gastrointestinal toxicity. Therefore, we conducted a case-control study across four regions in China to investigate this relationship. Inflammatory bowel disease (IBD) cases (219) were matched with healthy controls (438) based on age and gender and conditional logistic regression models and Restricted cubic splines were used to evaluate the association between MC-LR exposure and IBD risk. We used quantitative real-time polymerase chain reaction to measure the expression levels of inflammatory factors. The levels of protein expression in the colorectum were determined using Western blotting (WB). Compared to the lowest quartile of serum MC-LR levels, the adjusted odds ratios and 95% confidence intervals (CI) for the highest quartiles of serum MC-LR levels were 5.51 (2.70, 11.21). The RCS was shown the association between serum MC-LR levels and IBD risk was nonlinear ( < 0.001). In the animal experiments, MC-LR resulted in colorectal injury via the PI3K/AKT signaling pathway. Our study provides the evidence that serum MC-LR exposure is significantly associated with the risk of IBD in China. Animal study results indicate that MC-LR probably causes IBD via the PI3K/AKT signaling pathway.
微囊藻毒素-LR（MC-LR）是有害蓝藻水华产生的最普遍、种类最多的蓝藻毒素，与胃肠道毒性有关。因此，我们在中国四个地区进行了一项病例对照研究，以调查这种关系。根据年龄和性别，将炎症性肠病（IBD）病例（219例）与健康对照（438例）进行匹配，并使用条件逻辑回归模型和受限立方样条来评估MC-LR暴露与IBD风险之间的关联。我们使用定量实时聚合酶链反应来测量炎症因子的表达水平。使用蛋白质印迹法（WB）测定结肠直肠中的蛋白质表达水平。与血清MC-LR水平最低四分位数相比，血清MC-LR水平最高四分位数的调整优势比和95%置信区间（CI）为5.51（2.70，11.21）。受限立方样条显示血清MC-LR水平与IBD风险之间的关联是非线性的（<0.001）。在动物实验中，MC-LR通过PI3K/AKT信号通路导致结肠直肠损伤。我们的研究提供了证据，表明在中国，血清MC-LR暴露与IBD风险显著相关。动物研究结果表明，MC-LR可能通过PI3K/AKT信号通路导致IBD。
