Department of Small Animal Internal Medicine, Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel.
J Vet Intern Med. 2024 Nov-Dec;38(6):3105-3110. doi: 10.1111/jvim.17230. Epub 2024 Oct 30.
Oxidative stress is a potential contributor to chronic kidney disease (CKD) progression but has not been evaluated in dogs and cats with CKD.
Oxidative stress is higher in animals with CKD compared with healthy controls and decreases with the advancing CKD stage. The aim of this study was to determine the presence and intensity of oxidative stress in dogs and cats at different CKD stages.
Sixty dogs and 30 cats with naturally acquired CKD; 10 dogs and 14 cats, healthy controls.
Analytical cross-sectional study. Oxidative stress was evaluated by measuring the urinary concentration of F2-isoprostane (uF2-IsoPs) normalized to urinary creatinine.
Urinary F2-isoprostanes normalized to urinary creatinine of healthy dogs and of dogs with CKD Stages 1 to 4 was 3.3 ng/mg, 4.7 ng/mg (range, 1.0-73.4), 2.4 ng/mg (range, 0.4-7.8), 0.52 ng/mg (range, 0.01-2.9), and 0.37 ng/mg (range, 0.01-0.6), respectively. Urinary F2-isoprostanes differed among CKD stages (P < .001), but not compared with controls. uF2-IsoPs of healthy cats and cats with CKD Stages 1 to 4 was 0.68 ng/mg (range, 0.2-1.4), 0.97 ng/mg (range, 0.4-1.8), 0.6 ng/mg (range, 0.002-2.0), 0.94 ng/mg (range, 0.3-2.3), and 0.2 ng/mg (range, 0.01-0.4). Urinary F2-isoprostanes differed among stages (P = .05) but not compared with healthy controls.
Oxidative stress might be present in dogs and cats with CKD. Its magnitude declines as the disease progresses, therefore, it should be considered a potential therapeutic target mostly at the early stages of the disease.
氧化应激是慢性肾脏病（CKD）进展的潜在因素，但尚未在患有 CKD 的犬和猫中进行评估。
与健康对照组相比，患有 CKD 的动物的氧化应激水平更高，并且随着 CKD 阶段的进展而降低。本研究的目的是确定不同 CKD 阶段的犬和猫的氧化应激的存在和强度。
60 只患有自然获得性 CKD 的犬和 30 只猫；10 只犬和 14 只猫，健康对照组。
分析性横断面研究。通过测量尿肌酐标准化的 F2-异前列腺素（uF2-IsoPs）来评估氧化应激。
健康犬和 CKD 1 至 4 期犬的尿 F2-异前列腺素/尿肌酐分别为 3.3ng/mg、4.7ng/mg（范围，1.0-73.4）、2.4ng/mg（范围，0.4-7.8）、0.52ng/mg（范围，0.01-2.9）和 0.37ng/mg（范围，0.01-0.6）。CKD 各阶段之间的尿 F2-异前列腺素存在差异（P<.001），但与对照组无差异。健康猫和 CKD 1 至 4 期猫的 uF2-IsoPs 分别为 0.68ng/mg（范围，0.2-1.4）、0.97ng/mg（范围，0.4-1.8）、0.6ng/mg（范围，0.002-2.0）、0.94ng/mg（范围，0.3-2.3）和 0.2ng/mg（范围，0.01-0.4）。各阶段之间的尿 F2-异前列腺素存在差异（P=.05），但与健康对照组无差异。
氧化应激可能存在于患有 CKD 的犬和猫中。随着疾病的进展，其程度降低，因此，它应该被认为是疾病早期的一个潜在治疗靶点。
