Jiang Jian, Sun Cuiling, Wang Guanghui, Xu Qinnan, Bian Yuting, Li Jie, Li Jingdian, Ding Rong, Lin Houwen, Tian Wenjing, Chen Haifeng
Chen Fujian Provincial Key Laboratory of Innovative Drug Target, School of Pharmaceutical Sciences, Xiamen University, Xiamen, 361102, People's Republic of China.
State Key Laboratory of Oncogene and Related Genes, Department of Pharmacy, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, People's Republic of China.
Chem Biodivers. 2025 Jan;22(1):e202401778. doi: 10.1002/cbdv.202401778. Epub 2024 Oct 30.
Lotustine A (1), an undescribed C- norisoprenoid, along with 22 known analogues and two eudesmanoids, were isolated from the aerial parts of Nelumbo nucifera Gaertn. Among them, compounds 2, 15, 17, 21, 22, 24, 25 were isolated from N. nucifera leaves for the first time. Their structures, including absolute configurations, were elucidated by nuclear magnetic resonance, mass spectroscopy, and the modified Mosher's method. Compound 1 is the first example of C- norisoprenoid with a terminal double bond between C-5 and C-13. Moreover, the lipid-lowering activities of the isolates were evaluated, and the results showed that 2, 24 and 25 could remarkably decrease the levels of both total cholesterol and triglyceride in free fatty acids induced HepG2 cells at the concentration of 20 μM. The oil red staining assay further demonstrated the lipid-lowering effects of 2, 24 and 25. The western blot results indicated that compounds 2, 24 and 25 could regulate the lipid metabolism via the activation of the AMPK/ACC/SREBP-1c signaling pathway.
从莲（Nelumbo nucifera Gaertn.）的地上部分分离出了莲汀A（1），一种未描述的C-去甲异戊二烯类化合物，以及22种已知类似物和两种桉烷类化合物。其中，化合物2、15、17、21、22、24、25首次从莲叶中分离得到。通过核磁共振、质谱和改良的莫舍方法阐明了它们的结构，包括绝对构型。化合物1是在C-5和C-13之间具有末端双键的C-去甲异戊二烯类化合物的首个实例。此外，对分离物的降脂活性进行了评估，结果表明，在20 μM浓度下，化合物2、24和25可显著降低游离脂肪酸诱导的HepG2细胞中的总胆固醇和甘油三酯水平。油红染色试验进一步证明了化合物2、24和25的降脂作用。蛋白质印迹结果表明，化合物2、24和25可通过激活AMPK/ACC/SREBP-1c信号通路来调节脂质代谢。
