Department of Physical Education and Sport Sciences, National Taiwan Normal University, Taipei, TAIWAN.
School of Health and Exercise Sciences, University of British Columbia Okanagan, Kelowna, BC, CANADA.
Med Sci Sports Exerc. 2024 Nov 1;56(11):2117-2124. doi: 10.1249/MSS.0000000000003503.
Changes in plasma concentrations of anti-inflammatory cytokines, such as interleukin-6 (IL-6) and IL-10, after acute resistance exercise (RE) have been widely explored. Whether observed changes in plasma cytokine concentration correspond to the activation of anti-inflammatory signaling pathways in immune cells after acute RE is unknown. This study aimed to determine if changes in plasma cytokines after acute RE resulted in the activation of anti-inflammatory signaling pathways in peripheral blood mononuclear cells (PBMC).
Healthy young males (N = 16; age = 23.5 ± 2.7 yr; BMI = 22.4 ± 1.7 kg·m-2) participated in a single session of whole-body RE (4 sets of 4 different exercises at 70% 1-repetition maximum with the last set to failure) and a sedentary control (CON) condition in a randomized crossover design. Blood samples were collected at several time points before and after the exercise bout.
Higher plasma IL-6, IL-10, and IL-1 RA concentrations were observed after RE compared with CON. Phosphorylation of STAT3 and protein expression of SOCS3 in PBMC were increased in RE compared with CON. The elevation of plasma IL-6, but not IL-10, coincided with the activation of STAT3 signaling in PBMC.
These results highlight a potential mechanism by which RE may exert anti-inflammatory actions in circulating immune cells.
急性抗阻运动（RE）后，抗炎细胞因子（如白细胞介素-6（IL-6）和 IL-10）的血浆浓度变化已得到广泛研究。但急性 RE 后，观察到的细胞因子浓度变化是否对应于免疫细胞中抗炎信号通路的激活尚不清楚。本研究旨在确定急性 RE 后血浆细胞因子的变化是否导致外周血单个核细胞（PBMC）中抗炎信号通路的激活。
健康年轻男性（N=16；年龄=23.5±2.7 岁；BMI=22.4±1.7kg·m-2）采用随机交叉设计，分别进行一次全身性 RE（4 组，每组 4 种不同运动，强度为 70%1 次最大重复，最后一组至力竭）和安静对照（CON）条件。在运动前后的几个时间点采集血样。
与 CON 相比，RE 后血浆 IL-6、IL-10 和 IL-1RA 浓度升高。与 CON 相比，RE 时 PBMC 中 STAT3 的磷酸化和 SOCS3 的蛋白表达增加。血浆 IL-6 的升高与 PBMC 中 STAT3 信号的激活相吻合，但 IL-10 没有。
这些结果强调了 RE 可能通过循环免疫细胞发挥抗炎作用的潜在机制。
