Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Alquwayiyah, Riyadh, Saudi Arabia.
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
PLoS One. 2024 Oct 30;19(10):e0312590. doi: 10.1371/journal.pone.0312590. eCollection 2024.
Microorganisms in the gut and other niches may contribute to carcinogenesis while also altering cancer immune surveillance and therapeutic response. However, determining the impact of genetic variations and interplay with intestinal microbes' environment is difficult and unanswered. Here, we examined the frequency of thirteen mutant genes that caused aberrant gut in thirty different types of cancer using The Cancer Genomic Atlas (TCGA) database. Substantially, our findings show that all these mutated genes are quite frequent in uterine corpus endometrial cancer (UCEC). Further, these mutant genes are implicated in the infiltration of different subset of immune cells within the Tumor Microenvironment (TME) of UCEC patients. The top-ranking mutant genes that promote immune cell invasion into the TME of UCEC patients were PGLYRP2, OLFM4, and TLR5. In this regard, we used the same deconvolution of the TCGA database to analyze the microbiome that have a strong association with immune cells invasion with TME of UCEC patients. Several bacteria and viruses have been linked to the invasion of immune cells, such as B cell memory and T cell regulatory (Tregs), into the TME of UCEC patients. As a result, our findings pave the way for future research into generating novel immunizations against bacteria or viruses as immunotherapy for UCEC patients.
肠道和其他生态位中的微生物可能导致癌变，同时改变癌症的免疫监测和治疗反应。然而，确定遗传变异的影响以及与肠道微生物环境的相互作用是困难的，也是未解决的。在这里，我们使用癌症基因组图谱（TCGA）数据库检查了 30 种不同类型癌症中导致肠道异常的 13 种突变基因的频率。大量研究结果表明，所有这些突变基因在子宫体子宫内膜癌（UCEC）中都非常常见。此外，这些突变基因与 UCEC 患者肿瘤微环境（TME）中不同免疫细胞亚群的浸润有关。促进免疫细胞浸润 UCEC 患者 TME 的排名最高的突变基因是 PGLYRP2、OLFM4 和 TLR5。在这方面，我们使用相同的 TCGA 数据库去卷积来分析与 UCEC 患者 TME 中免疫细胞浸润有很强关联的微生物组。一些细菌和病毒与免疫细胞（如 B 细胞记忆和 T 细胞调节（Tregs））浸润 UCEC 患者的 TME 有关。因此，我们的研究结果为未来针对细菌或病毒的新型免疫接种作为 UCEC 患者的免疫疗法的研究铺平了道路。
