自衰变试剂对酶失活的动力学分析
Kinetic analysis of enzyme inactivation by an autodecaying reagent.
作者信息
Paquatte O, Tu S C
出版信息
Biochim Biophys Acta. 1986 Feb 14;869(3):359-62. doi: 10.1016/0167-4838(86)90076-2.
PMID:3947642
Abstract
A simple method is described for the determination of both the pseudo-first-order rate constant and the second-order rate constant for enzyme inactivation by a chemical reagent which itself undergoes exponential decay. The validity of this method has been demonstrated in two test cases in which the labile diethyl pyrocarbonate was used to inactivate salicylate hydroxylase and bacterial luciferase.
摘要
本文描述了一种简单的方法，用于测定一种本身呈指数衰减的化学试剂使酶失活的准一级速率常数和二级速率常数。该方法的有效性已在两个测试案例中得到证明，其中使用不稳定的焦碳酸二乙酯使水杨酸羟化酶和细菌荧光素酶失活。
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