College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; National Engineering Research Center for Fruit & Vegetable Processing, Key Laboratory of Fruit & Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory for Food Non-thermal Processing, China Agricultural University, Beijing 100083, China.
Gaoling Township, Baoding 071000, Hebei, China.
Food Chem. 2025 Feb 1;464(Pt 2):141594. doi: 10.1016/j.foodchem.2024.141594. Epub 2024 Oct 9.
Pepper seed protein isolate (PSPI) is a valuable plant-based protein source, yet the impact of processing methods such as high hydrostatic pressure (HHP) on its properties remains unclear. The impact of HHP on the structural and functional properties of PSPI at pH 7 and pH 9 was evaluated. Structural changes in PSPI were analyzed using spectral techniques, revealing significant alterations in the secondary and tertiary structures induced by HHP treatment. HHP treatment caused the unfolding of the PSPI structure, leading to the exposure of previously hidden chromophores and hydrophobic groups. The treatment also led to changes in free sulfhydryl groups and increased average particle size suggesting the formation of macromolecular polymers or insoluble aggregates. Consequently, the water-holding capacity, oil-holding capacity, foaming characteristics, and emulsifying activity index of the modified PSPI were significantly enhanced both at pH 7 and pH 9, with maximum improvements of 121.98 %, 157.29 %, 100.00 %, and 265.78 %, respectively. In conclusion, HHP is a promising strategy for enhancing the physicochemical properties of PSPI for various applications.
辣椒籽蛋白分离物（PSPI）是一种有价值的植物性蛋白质来源，但高压处理（HHP）等加工方法对其性质的影响尚不清楚。本研究评估了 HHP 在 pH 7 和 pH 9 下对 PSPI 结构和功能特性的影响。使用光谱技术分析 PSPI 的结构变化，结果表明 HHP 处理诱导了二级和三级结构的显著变化。HHP 处理导致 PSPI 结构展开，暴露出先前隐藏的发色团和疏水区。处理还导致游离巯基基团和平均粒径的变化，表明形成了高分子聚合物或不溶性聚集体。因此，改性 PSPI 在 pH 7 和 pH 9 时的持水能力、持油能力、泡沫特性和乳化活性指数均显著提高，分别最大提高了 121.98%、157.29%、100.00%和 265.78%。总之，HHP 是一种有前途的策略，可以增强 PSPI 的物理化学性质，从而应用于各种领域。
