利用基于CRISPR/Cas9的基因靶向技术生成SFTPC-mCherry敲入报告基因人胚胎干细胞系WAe001-A-2H。
Generation of SFTPC-mCherry knock-in reporter human embryonic stem cell line, WAe001-A-2H, using CRISPR/Cas9-based gene targeting.
作者信息
Sun Hui, Li Qian, Xu Tao, Zhang Wei, Sun Jiaqi, Liu Huisheng
机构信息
School of Biomedical Engineering, Guangzhou Medical University, Guangzhou 510180, China; Guangzhou National Laboratory, Guangzhou 510005, China.
Guangzhou National Laboratory, Guangzhou 510005, China.
出版信息
Stem Cell Res. 2024 Dec;81:103597. doi: 10.1016/j.scr.2024.103597. Epub 2024 Oct 28.
The SFTPC gene is responsible for the production of the pulmonary surfactant protein C (SPC), a highly hydrophobic molecule that plays a crucial role in maintaining lung integrity through its influence on the synthesis of alveolar surfactant proteins. In this study, we harnessed the CRISPR/Cas9 system for precise genome editing to create a modified H1 human embryonic stem cell (hESC) line, incorporating the SFTPC-mCherry reporter construct. Therefore, the engineered SFTPC-mCherry knock-in (KI) hESC line can serve as an effective tool for tracking the expression patterns of the SFTPC gene as alveolar type 2 cells differentiate from hESCs.
SFTPC基因负责产生肺表面活性物质蛋白C（SPC），这是一种高度疏水的分子，通过影响肺泡表面活性物质蛋白的合成，在维持肺的完整性方面发挥关键作用。在本研究中，我们利用CRISPR/Cas9系统进行精确的基因组编辑，创建了一个整合了SFTPC-mCherry报告基因构建体的修饰H1人类胚胎干细胞（hESC）系。因此，工程化的SFTPC-mCherry敲入（KI）hESC系可作为一种有效的工具，用于追踪SFTPC基因在2型肺泡细胞从hESC分化过程中的表达模式。
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