González-Espinosa Francisco, Di Pilato Vincenzo, Calabrese Luca, Costa Elisa, Costa Agustina, Gutkind Gabriel, Cejas Daniela, Radice Marcela
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Ciudad Autónoma de Buenos Aires, Argentina; CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Ciudad Autónoma de Buenos Aires, Argentina.
University of Genoa, Department of Surgical Sciences and Integrated Diagnostics (DISC), Genoa, Italy; IRCCS Ospedale Policlinico San Martino, Microbiology Unit, Genoa, Italy.
J Glob Antimicrob Resist. 2024 Dec;39:224-226. doi: 10.1016/j.jgar.2024.10.258. Epub 2024 Oct 29.
To characterise four bla-harbouring plasmids recovered in Enterobacterales isolated in Argentina.
DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and visualised by Proksee and Clinker. bla-harbouring plasmids were compared with similar deposited plasmids using PLSDB.
Two plasmids belonged to incompatibility group IncFII where bla was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥90% shared hashes (sh)), with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring bla in a novel variant of the genetic context. Both multi-replicon plasmids presented two ARIs, one containing bla in addition to another antimicrobial resistance marker (ARM), and the second ARI carrying bla and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (n = 5) displayed less than 60% shared hashes and showed the same Inc groups but lacked ARM.
This study broadens the limited understanding of bla-harbouring plasmids in Latin America. Furthermore, it represents the first description of bla in a novel variant of common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any ARM.
对在阿根廷分离出的肠杆菌科细菌中获得的四种携带bla的质粒进行特征分析。
采用Illumina和牛津纳米孔技术对DNA进行测序，使用Unicycler进行组装，利用PlasmidFinder、MOB-Typer、IslandViewer4和Resfinder进行分析，并通过Proksee和Clinker进行可视化。使用PLSDB将携带bla的质粒与已存入的相似质粒进行比较。
两种质粒属于不相容群IncFII，其中bla位于先前描述的遗传背景中，处于一个抗微生物抗性岛（ARI）内。本地IncFII质粒显示出高度相似性（共享哈希值（sh）≥90%），与PLSDB中存入的四种质粒相似。另外两种本地质粒属于多复制子群IncFIB-HI1B，在一种新的遗传背景变体中携带bla。这两种多复制子质粒都有两个ARI，一个除了另一个抗微生物抗性标记（ARM）外还含有bla，第二个ARI携带bla和一个1类整合子。存入PLSDB的质粒与本地多复制子质粒的相似性较低。最相似的质粒（n = 5）共享哈希值小于60%，显示出相同的Inc群，但缺乏ARM。
本研究拓宽了对拉丁美洲携带bla质粒的有限认识。此外，它首次描述了bla在常见遗传平台新变体中的情况及其在多复制子IncFIB-IncHI1B质粒中的位置，这些质粒以前未与任何ARM相关联。
