School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan 430022, China.
School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan 430022, China.
Bone. 2025 Jan;190:117310. doi: 10.1016/j.bone.2024.117310. Epub 2024 Oct 28.
Neutrophils have been extensively studied for their critical roles in supporting immune defense mechanisms, initiating bone regeneration, and promoting angiogenesis. Nonetheless, the influence of neutrophils on physiological conditions, particularly in the context of bone development, remains incompletely understood. In this study, we examined the effects of non-inflammatory neutrophils on bone physiology by depleting Ly6G neutrophils and inducing neutropenia through myelosuppression. Our results demonstrated a notable increase in bone mass and a decrease in the bone marrow cavity upon depletion of the neutrophils. These effects were attributed to the direct interaction between neutrophils and osteoblasts, independent of reduced secretion of typical inflammatory cytokines or diminished osteoclast differentiation. This observation suggests a non-inflammatory function of neutrophils within the endosteal microenvironment, where they regulate osteogenic differentiation to preserve optimal bone mass, shape healthy three-dimensional bone trabecular structures, and create ample space for hematopoietic niche development.
中性粒细胞在支持免疫防御机制、启动骨再生和促进血管生成方面发挥着至关重要的作用，已经得到了广泛的研究。然而，中性粒细胞对生理状况的影响，特别是在骨发育方面的影响，仍不完全清楚。在这项研究中，我们通过耗竭 Ly6G 中性粒细胞和诱导骨髓抑制来研究中性粒细胞对骨生理学的影响。我们的研究结果表明，在耗竭中性粒细胞后，骨量显著增加，骨髓腔减小。这些影响归因于中性粒细胞与成骨细胞之间的直接相互作用，而与减少典型炎症细胞因子的分泌或减少破骨细胞分化无关。这一观察结果表明中性粒细胞在骨内膜微环境中具有非炎症性功能，它们调节成骨细胞分化以维持最佳骨量，形成健康的三维骨小梁结构，并为造血龛的发育创造充足的空间。
