右美托咪定和左旋布比卡因对实验性缺血再灌注模型的影响。
The Effect of Dexmedetomidine and Levobupivacaine in an Experimental Ischemia Reperfusion Model.
机构信息
Department of Anesthesia and Reanimation, Health Science University, Hamidiye Etfal, Training, and Research Hospital, Istanbul, Turkey.
Department of Anesthesia and Reanimation, Health Science University, Hamidiye Etfal, Training, and Research Hospital, Istanbul, Turkey;
出版信息
In Vivo. 2024 Nov-Dec;38(6):2696-2704. doi: 10.21873/invivo.13747.
BACKGROUND/AIM: Although it has been reported that different molecules are effective in preventing ischemia-reperfusion (I/R) injury, the most effective treatment is still unknown.
MATERIALS AND METHODS
The rats were divided into four groups of eight rats each. Group C: 1 ml intraperitoneal (IP) isotonic + laparotomy + IP 2 ml isotonic +I/R. Group D: 100 μg kg/1 ml IP dexmedetomidine + laparotomy + IP 2 ml isotonic +I/R. Group L: 1 ml IP isotonic + laparotomy + IP levobupivacaine (2.5 mg kg/2 ml) +I/R. Group DL: 100 μg kg/1 ml IP dexmedetomidine + laparotomy + IP levobupivacaine (2.5 mg kg/2 ml) +I/R. Brain, heart, lung, and liver tissue samples were collected for histopathological examination. Biochemically, levels of aspartate amino transaminase, alanine amino transaminase, serum glucose, total antioxidant status (TAS), total oxidant status, ischemia modified albumin, and malondialdehyde were measured in blood samples.
RESULTS
Group D mean blood TAS levels were found to be statistically significantly higher than those in Group C and Group L (p=0.037, p=0.048 respectively). Group DL oxidative stress index (OSI) value was found to be statistically significantly lower than that of Group C (p=0.010).
CONCLUSION
Both dexmedetomidine and levobupivacaine demonstrated protective effects in I/R injury. When used in combination, the effects of these treatments were further enhanced, reaching statistical significance. As our literature review found no studies on the combined use of dexmedetomidine and levobupivacaine in I/R injury, it is anticipated that supporting these results with clinical studies may significantly contribute to clinical practice.
背景/目的：虽然已经有报道称不同的分子在预防缺血再灌注（I/R）损伤方面有效，但最有效的治疗方法仍不清楚。
材料和方法
将大鼠分为四组，每组 8 只。C 组：腹腔内（IP）注射 1 ml 等渗盐水+剖腹术+IP 注射 2 ml 等渗盐水+I/R。D 组：腹腔内注射 100 μg/kg/1 ml 右美托咪定+剖腹术+IP 注射 2 ml 等渗盐水+I/R。L 组：腹腔内注射 1 ml 等渗盐水+剖腹术+IP 注射左旋布比卡因（2.5 mg/kg/2 ml）+I/R。DL 组：腹腔内注射 100 μg/kg/1 ml 右美托咪定+剖腹术+IP 注射左旋布比卡因（2.5 mg/kg/2 ml）+I/R。收集脑、心、肺和肝组织样本进行组织病理学检查。测定血样中天冬氨酸氨基转移酶、丙氨酸氨基转移酶、血糖、总抗氧化状态（TAS）、总氧化状态、缺血修饰白蛋白和丙二醛的水平。
结果
与 C 组和 L 组相比，D 组的平均血液 TAS 水平显著升高（p=0.037，p=0.048）。DL 组的氧化应激指数（OSI）值显著低于 C 组（p=0.010）。
结论
右美托咪定和左旋布比卡因在 I/R 损伤中均表现出保护作用。当两者联合使用时，这些治疗的效果进一步增强，达到统计学意义。由于我们的文献综述没有发现关于右美托咪定和左旋布比卡因联合应用于 I/R 损伤的研究，因此预计通过临床研究支持这些结果可能会对临床实践有重大贡献。
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