Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Nat Commun. 2024 Oct 30;15(1):9380. doi: 10.1038/s41467-024-53579-6.
Phototherapy can offer a safe and non-invasive solution against infections, while promoting wound healing. Conventional phototherapeutic devices are bulky and limited to hospital use. To overcome these challenges, we developed a wearable, flexible red and blue LED (r&bLED) patch controlled by a mobile-connected system, enabling safe self-application at home. The patch exhibits excellent skin compatibility, flexibility, and comfort, with high safety under system supervision. Additionally, we synthesized a sprayable fibrin gel (F-gel) containing blue light-sensitive thymoquinone and red light-synergistic NADH. Combined with bLED, thymoquinone eradicated microbes and biofilms within minutes, regardless of antibiotic resistance. Furthermore, NADH and rLED synergistically improved macrophage and endothelial cell mitochondrial function, promoting wound healing, reducing inflammation, and enhancing angiogenesis, as validated in infected diabetic wounds in mice and minipigs. This innovative technology holds great promise for revolutionizing at-home phototherapy for chronic infected wounds.
光疗可以提供一种安全、非侵入性的感染解决方案，同时促进伤口愈合。传统的光疗设备体积庞大，仅限于在医院使用。为了克服这些挑战，我们开发了一种由移动连接系统控制的可穿戴、灵活的红色和蓝色 LED(r&bLED)贴片，可在家中安全地进行自我应用。该贴片具有出色的皮肤相容性、灵活性和舒适性，在系统监管下具有高度安全性。此外，我们合成了一种可喷涂的纤维蛋白凝胶（F-gel），其中含有对蓝光敏感的百里醌和红光协同 NADH。与 bLED 结合使用，百里醌能在数分钟内消除微生物和生物膜，无论是否存在抗生素耐药性。此外，NADH 和 rLED 协同作用可改善巨噬细胞和内皮细胞的线粒体功能，促进伤口愈合，减少炎症，增强血管生成，这在小鼠和小型猪的感染性糖尿病伤口中得到了验证。这项创新技术有望彻底改变慢性感染性伤口的家庭光疗。
