Department of Genetics and Marine Biotechnology, Institute of Oceanology, Polish Academy of Sciences, Powstańców Warszawy 55 Str., 81-712, Sopot, Poland.
Sci Data. 2024 Oct 30;11(1):1184. doi: 10.1038/s41597-024-04004-6.
Although the European flounder is frequently used in research and has economic importance, there is still lack of comprehensive transcriptome data for this species. In the present research we show RNA-Seq data from ten selected organs of P. flesus female inhabiting brackish waters of the Gulf of Gdańsk (southern Baltic Sea). High throughput Next Generation Sequencing technology NovaSeq 6000 was used to generate 500 M sequencing reads. These were mapped against European flounder reference genome and reads extracted from the mapping were assembled producing 61k reliable contigs. Gene ontology (GO) terms were assigned to the majority of annotated contigs/unigenes based on the results of PFAM, PANTHER, UniProt and InterPro protein databases searches. BUSCOs statistics for eukaryota, metazoa, vertebrata and actinopterygii databases showed that the reported transcriptome represents a high level of completeness. The data set can be successfully used as a tool in design of experiments from various research fields including biology, aquaculture and toxicology.
尽管欧牙鲆经常被用于研究且具有重要的经济价值，但该物种仍缺乏全面的转录组数据。在本研究中，我们展示了来自栖息于加迪夫湾（波罗的海南部）的雌性欧牙鲆十种选定器官的 RNA-Seq 数据。我们使用高通量下一代测序技术 NovaSeq 6000 生成了 5000 万条测序reads。这些 reads 被映射到欧洲牙鲆参考基因组上，并从映射中提取 reads 进行组装，生成了 61k 个可靠的 contigs。根据 PFAM、PANTHER、UniProt 和 InterPro 蛋白质数据库搜索的结果，为大多数注释的 contigs/基因赋予了基因本体论 (GO) 术语。真核生物、后生动物、脊椎动物和硬骨鱼纲数据库的 BUSCOs 统计显示，所报道的转录组具有较高的完整性水平。该数据集可成功用作包括生物学、水产养殖和毒理学在内的各个研究领域的实验设计工具。
