School of Stomatology, Zunyi Medical University, Zunyi, China.
Department of Stomatology, Luoyang Maternal and Child Health Hospital, Luoyang, China.
Sci Rep. 2024 Oct 30;14(1):26135. doi: 10.1038/s41598-024-75941-w.
The global aging population has led to a rise in age-related health issues, such as malnutrition, metabolic disorders, and even immune decline. Among these concerns, periodontitis holds particular significance for the well-being of the elderly. This study aimed to investigate the impact of aging on inflammatory resorption of alveolar bone in mice with periodontitis, with a specific focus on alterations in the intestinal microenvironment. To achieve this, we established a D-galactose (D-gal)-induced aging mouse model with periodontitis and employed histopathological staining, oxidative stress, and inflammatory factors analyses to assess the severity of periodontitis and the health status. Additionally, the 16S rRNA sequencing and untargeted metabolomics analysis were employed to investigate alterations in the intestinal microbiota and metabolites. Our results showed that D-gal-induced aging mice with periodontitis experienced more pronounced alveolar bone inflammatory resorption and disruptions in the gut barrier, accompanied by an overall decline in physical condition. The microbial composition and structure of aged mice also underwent significant modifications, with a decreased Firmicutes/Bacteroidetes (F/B) ratio. Furthermore, metabolomics analysis demonstrated that D-gal-induced aging primarily influenced lipids and lipid-like molecules metabolism, and enrichment observed in the rheumatoid arthritis and histidine metabolism pathways. These findings provide further evidence that the aging process exacerbates age-related alveolar bone loss (ABL) through disturbances in intestinal homeostasis.
全球人口老龄化导致与年龄相关的健康问题（如营养不良、代谢紊乱，甚至免疫下降）有所增加。在这些问题中，牙周炎对老年人的健康尤为重要。本研究旨在探讨衰老对牙周炎小鼠牙槽骨炎症吸收的影响，特别关注肠道微环境的变化。为此，我们建立了 D-半乳糖（D-gal）诱导的牙周炎衰老小鼠模型，通过组织病理学染色、氧化应激和炎症因子分析来评估牙周炎的严重程度和健康状况。此外，还进行了 16S rRNA 测序和非靶向代谢组学分析，以研究肠道微生物群和代谢物的变化。我们的结果表明，D-gal 诱导的牙周炎衰老小鼠经历了更明显的牙槽骨炎症吸收和肠道屏障破坏，同时整体身体状况下降。年老小鼠的微生物组成和结构也发生了显著改变，厚壁菌门/拟杆菌门（Firmicutes/Bacteroidetes，F/B）比值降低。此外，代谢组学分析表明，D-gal 诱导的衰老主要影响脂质和类脂分子代谢，并且在类风湿关节炎和组氨酸代谢途径中观察到富集。这些发现进一步证明，衰老过程通过肠道内稳态的破坏加剧了与年龄相关的牙槽骨丢失（ABL）。
