Click Chemistry for Visualization of Newly Synthesized RNA and Antibody Labeling on Ultrathin Tissue Sections.
作者信息
Pérez-Garza Janeth, Orea Jairo, Ostroff Linnaea
机构信息
Department of Physiology and Neurobiology. University of Connecticut, Storrs, CT.
Connecticut Institute for the Brain and Cognitive Sciences, University of Connecticut, Storrs, CT.
出版信息
Microsc Microanal. 2023 Jul 22;29(29 Suppl 1):1075-1076. doi: 10.1093/micmic/ozad067.552.
Abstract
摘要
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本文引用的文献
1
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Acta Neuropathol Commun. 2021 May 21;9(1):94. doi: 10.1186/s40478-021-01200-y.
2
A Hitchhiker's Guide to Click-Chemistry with Nucleic Acids.
Chem Rev. 2021 Jun 23;121(12):7122-7154. doi: 10.1021/acs.chemrev.0c00928. Epub 2021 Jan 14.
3
Expanding the Scope of RNA Metabolic Labeling with Vinyl Nucleosides and Inverse Electron-Demand Diels-Alder Chemistry.
ACS Chem Biol. 2019 Aug 16;14(8):1698-1707. doi: 10.1021/acschembio.9b00079. Epub 2019 Jul 25.
4
The travels of mRNAs in neurons: do they know where they are going?
Curr Opin Neurobiol. 2019 Aug;57:110-116. doi: 10.1016/j.conb.2019.01.016. Epub 2019 Feb 19.
5
Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo.
Nat Protoc. 2019 Feb;14(2):556-575. doi: 10.1038/s41596-018-0106-6.
6
Global analysis of RNA metabolism using bio-orthogonal labeling coupled with next-generation RNA sequencing.
Methods. 2019 Feb 15;155:88-103. doi: 10.1016/j.ymeth.2018.12.001. Epub 2018 Dec 6.
7
Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria.
Elife. 2018 Sep 10;7:e37243. doi: 10.7554/eLife.37243.
8
Visualization of newly synthesized neuronal RNA in vitro and in vivo using click-chemistry.
RNA Biol. 2017 Jan 2;14(1):20-28. doi: 10.1080/15476286.2016.1251541. Epub 2016 Nov 1.
9
The central dogma decentralized: new perspectives on RNA function and local translation in neurons.
Neuron. 2013 Oct 30;80(3):648-57. doi: 10.1016/j.neuron.2013.10.036.
10
Click Chemistry: Diverse Chemical Function from a Few Good Reactions.
Angew Chem Int Ed Engl. 2001 Jun 1;40(11):2004-2021. doi: 10.1002/1521-3773(20010601)40:11<2004::AID-ANIE2004>3.0.CO;2-5.
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