Zhao Yuyang, Chen Zhimin, Dong Ruiyi, Liu Yufan, Zhang Yixin, Guo Yan, Yu Meiyi, Li Xiang, Wang Jiangbin
Department of Gastroenterology China-Japan Union Hospital of Jilin University Changchun Jilin China.
Department of Pharmacology College of Basic Medical Sciences, Jilin University Changchun Jilin China.
Food Sci Nutr. 2024 Aug 30;12(10):8309-8323. doi: 10.1002/fsn3.4426. eCollection 2024 Oct.
A high-fat diet (HFD) is recognized as an important contributor to inflammatory bowel disease (IBD). However, the precise underlying mechanism of HFD on IBD remains elusive. This study aimed to investigate the potential mechanism by which HFD affects IBD using 16S rRNA-sequencing and RNA-seq technology. Results indicated that HFD-treated mice exhibited notable alternations in the structure and composition of the gut microbiota, with some of these alternations being associated with the pathogenesis of IBD. Analysis of the colon transcriptome revealed 11 hub genes and 7 hub pathways among control, DSS-induced colitis, and HFD + DSS-treated groups. Further analysis explores the relationship between the hub pathways and genes, as well as the hub genes and gut microbiota. Overall, the findings indicate that the impact of HFD on DSS-induced colitis may be linked to intestinal dysbiosis and specific genes such as , , , , , , , , , , and . These results provide valuable insights for determining potential therapeutic targets for addressing HFD-induced IBD.
高脂饮食（HFD）被认为是炎症性肠病（IBD）的一个重要促成因素。然而，HFD对IBD的确切潜在机制仍不清楚。本研究旨在利用16S rRNA测序和RNA测序技术探究HFD影响IBD的潜在机制。结果表明，经HFD处理的小鼠肠道微生物群的结构和组成出现显著变化，其中一些变化与IBD的发病机制有关。对结肠转录组的分析揭示了对照组、DSS诱导的结肠炎组和HFD + DSS处理组中的11个枢纽基因和7条枢纽通路。进一步分析探讨了枢纽通路与基因之间的关系，以及枢纽基因与肠道微生物群之间的关系。总体而言，研究结果表明，HFD对DSS诱导的结肠炎的影响可能与肠道生态失调以及特定基因如 、 、 、 、 、 、 、 、 、 和 有关。这些结果为确定解决HFD诱导的IBD的潜在治疗靶点提供了有价值的见解。
