Wang Ting, Xu Gaoli, Zhang Chuankai, Forouzanfar Tymour, Liang Junwei, Pan Yulei, Shen Chenxi, Wu Gang, Lin Haiyan
Department of Oral and Maxillofacial Surgery, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
School/Hospital of Stomatology, Zhejiang Chinese Medical University, Hangzhou, China.
Clin Implant Dent Relat Res. 2025 Feb;27(1):e13408. doi: 10.1111/cid.13408. Epub 2024 Oct 31.
To evaluate the efficacy of 3D-printed scaffolds that were osteoinductively functionalized with a bone morphogenetic protein 2 (BMP-2)-incorporated biomimetic calcium phosphate particles (BMP-2-inc. BpNcCaP)/hyaluronic acid (HA) composite gel in vertical bone augmentation in beagle dogs.
Four Beagle dogs were used in this study. Three months after the extraction of 1st, 2nd, 3rd, and 4th premolars at both sides of the lower jaws of Beagle dogs, one or two critical-size vertical bone defects (4 mm vertical bone defect without buccal and lingual bone) on each side were surgically created. The defects were randomly subjected to the following groups: (1) Control (without bone-defect-filling materials); (2) 3D scaffold; (3) BMP2-inc. BpNcCaP/HA-functionalized 3D scaffold. Six weeks post-surgery, samples were harvested and subjected to micro-CT and histomorphometric analyses.
The struts of the BMP2-inc. BpNcCaP/HA-func. 3D scaffold were covered by a thick layer of cemented irregular particles with an average pore size at 327 ± 27 μm. The BpNcCaP/HA-func. 3D scaffold group bore significantly higher bone volume, bone volume fraction, trabecular number, trabecular thickness, bone mineral density, connectivity density, and bone volumes in three directions (mesiodistal, buccolingual, and apicocoronal) when compared with the groups of Control and 3D scaffold. Moreover, the BMP2-inc. BpNcCaP/HA-func. 3D scaffold group bore significantly lower trabecular separation and exhibited significantly higher bone-to-scaffold contact percentage and newly formed bone area percentage within pores in comparison with 3D scaffold.
BMP2-inc. BpNcCaP/HA-func. 3D scaffold dramatically enhanced vertical alveolar bone augmentation, which suggests a promising application potential of BMP2-inc. BpNcCaP/HA-func. 3D scaffold in dental clinic.
评估用掺入骨形态发生蛋白2（BMP-2）的仿生磷酸钙颗粒（BMP-2-inc. BpNcCaP）/透明质酸（HA）复合凝胶进行骨诱导功能化的3D打印支架在比格犬垂直骨增量中的疗效。
本研究使用了4只比格犬。在比格犬下颌两侧拔除第1、2、3和4前磨牙3个月后，在每侧手术制造1个或2个临界尺寸的垂直骨缺损（4毫米垂直骨缺损，无颊侧和舌侧骨）。将缺损随机分为以下几组：（1）对照组（无骨缺损填充材料）；（2）3D支架组；（3）BMP2-inc. BpNcCaP/HA功能化3D支架组。术后6周，采集样本并进行显微CT和组织形态计量分析。
BMP2-inc. BpNcCaP/HA功能化3D支架的支柱被一层厚厚的胶结不规则颗粒覆盖，平均孔径为327±27μm。与对照组和3D支架组相比，BpNcCaP/HA功能化3D支架组在骨体积、骨体积分数、小梁数量、小梁厚度、骨矿物质密度、连通性密度以及三个方向（近远中、颊舌、根尖冠向）的骨体积方面均显著更高。此外，与3D支架组相比，BMP2-inc. BpNcCaP/HA功能化3D支架组的小梁间距显著更低，骨与支架的接触百分比以及孔隙内新形成骨面积百分比显著更高。
BMP2-inc. BpNcCaP/HA功能化3D支架显著增强了垂直牙槽骨增量，这表明BMP2-inc. BpNcCaP/HA功能化3D支架在牙科临床具有广阔的应用潜力。
