Fruit and Tea Research Institute, Hubei Academy of Agricultural Sciences, Wuhan, Hubei, China.
Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Wuhan, Hubei, China.
Physiol Plant. 2024 Nov-Dec;176(6):e14597. doi: 10.1111/ppl.14597.
Red peel color, a desirable trait in pears, is determined by anthocyanin accumulation. While REVEILLE (RVEs) transcription factors regulate anthocyanin biosynthesis in some plant species, their role in pear peel has not been well-studied. This study investigates the function of RVEs in anthocyanin biosynthesis in 'Zaosu' (low anthocyanin content) and its red bud mutant, 'Red Zaosu' (high anthocyanin content) fruit peel. Consistent with higher anthocyanin content, 'Red Zaosu' pears exhibited increased PbRVE6 expression compared to 'Zaosu', while PbRVE3a and PbRVE3b levels remained unchanged. Additionally, PbRVE6 was localized to the nucleus. Overexpression of PbRVE6 in 'Zaosu' pear pericarp significantly increased anthocyanin content and upregulated key anthocyanin pathway genes PbANS and PbUFGT. Conversely, VIGS silencing of PbRVE6 in 'Red Palacer' pears led to decreased expression of PbANS and PbUFGT and a slight reduction in anthocyanin content. Yeast one-hybrid and dual-luciferase assays confirmed that PbRVE6 can bind and activate the promoters of PbANS and PbUFGT. These findings demonstrate that PbRVE6 promotes anthocyanin accumulation in pear peel by directly regulating PbANS and PbUFGT expression. This study provides a valuable foundation for understanding the regulatory network of anthocyanins in pear peels, and offers potential genetic resources for the production of new pear germplasm.
果皮红色是梨的一个理想性状，由花青苷积累决定。虽然 REVEILLE（RVEs）转录因子在一些植物物种中调节花青苷生物合成，但它们在梨果皮中的作用尚未得到很好的研究。本研究调查了 RVEs 在 'Zaosu'（低花青苷含量）及其红色芽变 'Red Zaosu'（高花青苷含量）果皮中花青苷生物合成中的功能。与较高的花青苷含量一致，'Red Zaosu' 梨中 PbRVE6 的表达水平明显高于 'Zaosu'，而 PbRVE3a 和 PbRVE3b 水平保持不变。此外，PbRVE6 定位于细胞核。在 'Zaosu' 梨果皮中过表达 PbRVE6 显著增加了花青苷含量，并上调了关键花青苷途径基因 PbANS 和 PbUFGT。相反，在 'Red Palacer' 梨中 VIGS 沉默 PbRVE6 导致 PbANS 和 PbUFGT 的表达下调，花青苷含量略有降低。酵母单杂交和双荧光素酶报告基因分析证实，PbRVE6 可以结合并激活 PbANS 和 PbUFGT 的启动子。这些发现表明，PbRVE6 通过直接调节 PbANS 和 PbUFGT 的表达促进了梨果皮中花青苷的积累。本研究为理解梨果皮中花青苷的调控网络提供了有价值的基础，并为生产新的梨种质资源提供了潜在的遗传资源。
