Zhang Tingrui, Tai Zongguang, Miao Fengze, Zhao Yingchao, Wang Weifan, Zhu Quangang, Chen Zhongjian
School of Medicine, Shanghai University, Shanghai 200444, China.
Shanghai Skin Disease Hospital, Tongji University School of Medicine, 1278 Baode Road, Shanghai 200443, P. R. China.
J Mater Chem B. 2024 Dec 4;12(47):12338-12348. doi: 10.1039/d3tb02158k.
Macrophages play a crucial role in the process of wound healing. In order to effectively inhibit excessive inflammation and facilitate skin wound healing, it is necessary to transform overactive M1 macrophages in injured tissues into the M2 type. In this study, we have successfully generated bioinspired nanovesicles (referred to as M2BNVs) from M2 type macrophages. These nanovesicles not only possess physical and biological properties that closely resemble exosomes, but also offer a simpler preparation process and more abundant yield. Owing to their distinctive endogenous cargo, M2BNVs have the ability to re-educate M1 macrophages, shifting their phenotype towards the M2 type which is known to promote healing and possess anti-inflammatory properties. Consequently, M2BNVs effectively improve the prevailing pro-inflammatory microenvironment within the wound. Furthermore, M2BNVs also facilitate wound tissue regeneration and angiogenesis. Collectively, our findings demonstrate the potential of M2BNVs in promoting wound healing in mice.
巨噬细胞在伤口愈合过程中发挥着关键作用。为了有效抑制过度炎症并促进皮肤伤口愈合，有必要将受损组织中过度活跃的M1巨噬细胞转化为M2型。在本研究中，我们成功地从M2型巨噬细胞中生成了仿生纳米囊泡（称为M2BNVs）。这些纳米囊泡不仅具有与外泌体极为相似的物理和生物学特性，而且制备过程更简单、产量更丰富。由于其独特的内源性物质，M2BNVs能够重塑M1巨噬细胞，使其表型向已知可促进愈合并具有抗炎特性的M2型转变。因此，M2BNVs有效地改善了伤口内普遍存在的促炎微环境。此外，M2BNVs还促进伤口组织再生和血管生成。总的来说，我们的研究结果证明了M2BNVs在促进小鼠伤口愈合方面的潜力。
