Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16(th) Ave., Gainesville, FL 32610, USA.
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16(th) Ave., Gainesville, FL 32610, USA.
Res Vet Sci. 2024 Nov;180:105441. doi: 10.1016/j.rvsc.2024.105441. Epub 2024 Oct 28.
Canine atopic dermatitis (AD) is a common skin disease. Many therapeutic options are available to decrease inflammation and ameliorate pruritus. However, those treatments are associated with side effects, a long lag phase for efficacy, or high expense. Natural plant extracts have been identified as possible, safer alternatives to traditional anti-inflammatory and antipruritic drugs. Resveratrol has been revisited as a new, possible alternative for its numerous beneficial properties. The purpose of this study was to evaluate the in vitro effects of resveratrol on peripheral blood mononuclear cells (PBMC) harvested from healthy and atopic privately-owned dogs. The PBMC harvested from nine healthy and 11 atopic dogs were isolated and exposed to four concentrations (1.5-9 μg/mL) of resveratrol both with or without phytohemagglutinin stimulation. After 24 h cytotoxicity, host defense peptides (HDPs), as well as oxidative stress (catalase and superoxide dismutase), and pro-inflammatory cytokines were assessed. Cytotoxicity was not observed in either group under any experimental conditions. An increase in catalase was only seen in healthy PBMC after exposure to low concentrations of resveratrol (p ≤ 0.03). Resveratrol did not show any effect on canine HDPs. Compared to baseline, there was a significant reduction in monocyte chemotactic protein-1 and interleukin-6 after exposure to 9 μg/mL of resveratrol in unstimulated healthy (p = 0.029) and stimulated atopic (p = 0.0075) PBMC. In conclusion, these data confirm the overall lack of cytotoxicity of resveratrol on healthy and atopic PBMC at the tested concentrations. However, at the concentrations tested, there was only a minimal effect of resveratrol on the secretion of HDPs and pro-inflammatory cytokines.
犬特应性皮炎（AD）是一种常见的皮肤病。有许多治疗选择可用于减轻炎症和改善瘙痒。然而，这些治疗方法与副作用、疗效的长滞后期或高费用有关。天然植物提取物已被确定为传统抗炎和止痒药物的更安全替代品。白藜芦醇因其众多有益特性而被重新视为一种新的可能替代品。本研究旨在评估白藜芦醇对来自健康和特应性私人拥有的犬的外周血单核细胞（PBMC）的体外影响。从 9 只健康犬和 11 只特应性犬中分离出 PBMC，并在有或没有植物血凝素刺激的情况下暴露于 4 种浓度（1.5-9μg/mL）的白藜芦醇。在 24 小时细胞毒性后，评估宿主防御肽（HDPs）、氧化应激（过氧化氢酶和超氧化物歧化酶）和促炎细胞因子。在任何实验条件下，两组均未观察到细胞毒性。仅在健康 PBMC 暴露于低浓度白藜芦醇后观察到过氧化氢酶增加（p≤0.03）。白藜芦醇对犬 HDPs 没有显示任何影响。与基线相比，在未刺激的健康（p=0.029）和刺激的特应性（p=0.0075）PBMC 中，暴露于 9μg/mL 白藜芦醇后，单核细胞趋化蛋白-1 和白细胞介素-6 的水平显著降低。总之，这些数据证实了在测试浓度下，白藜芦醇对健康和特应性 PBMC 总体上没有细胞毒性。然而，在测试的浓度下，白藜芦醇对白藜芦醇对 HDPs 和促炎细胞因子分泌的影响仅很小。
