Nuttall T J, Lamb J R, Hill P B
Easter Bush Veterinary Centre, University of Edinburgh Hospital for Small Animals, Easter Bush, Midlothian EH25 9RG, UK.
Vet Immunol Immunopathol. 2001 Oct;82(3-4):273-80. doi: 10.1016/s0165-2427(01)00361-0.
Atopic dermatitis is a chronic inflammatory and pruritic skin disease commonly seen in dogs and humans that is characterised by the presence of allergen-specific IgE. Data from skin tests and serological analysis suggest that the house dust mite Dermatophagoides farinae is the most important allergen in dogs with atopic dermatitis. The aim of this study was to determine if D. farinae specific peripheral blood mononuclear cell (PBMC) responses could be detected in dogs with atopic dermatitis. PBMCs were isolated by the density centrifugation from dogs with atopic dermatitis that were skin test positive for D. farinae, dogs with atopic dermatitis that were skin test negative for D. farinae, and healthy dogs. Cells were cultured with increasing concentrations of the D. farinae extract, no antigen, vaccine antigens or concanavalin A (ConA). There was significantly greater responsiveness of PBMCs from the D. farinae positive dogs than from either the D. farinae negative or healthy dogs (ANOVA, P<0.05). In contrast, no significant differences were observed in the control responses between the three groups. This is the first study to demonstrate that D. farinae specific circulating memory cells are involved in the pathogenesis of canine house dust mite hypersensitivity.
特应性皮炎是一种常见于犬类和人类的慢性炎症性瘙痒性皮肤病，其特征是存在过敏原特异性IgE。皮肤试验和血清学分析数据表明，屋尘螨是患有特应性皮炎犬类中最重要的过敏原。本研究的目的是确定在患有特应性皮炎的犬类中是否能检测到针对屋尘螨的外周血单核细胞（PBMC）反应。通过密度离心法从对屋尘螨皮肤试验呈阳性的特应性皮炎犬、对屋尘螨皮肤试验呈阴性的特应性皮炎犬以及健康犬中分离出PBMC。将细胞与浓度不断增加的屋尘螨提取物、无抗原、疫苗抗原或刀豆蛋白A（ConA）一起培养。屋尘螨阳性犬的PBMC反应性明显高于屋尘螨阴性犬或健康犬（方差分析，P<0.05）。相比之下，三组之间的对照反应未观察到显著差异。这是第一项证明针对屋尘螨的循环记忆细胞参与犬类屋尘螨超敏反应发病机制的研究。
