School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China; National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, 230036, China; Key Laboratory of Crop Stress Resistance and High Quality Biology of Anhui Province, Anhui Agricultural University, Hefei, 230036, China.
Inner Mongolia Academy of Science and Technology, Hohhot, 010010, Inner Mongolia, China.
Environ Res. 2024 Dec 15;263(Pt 3):120262. doi: 10.1016/j.envres.2024.120262. Epub 2024 Oct 29.
Keystone taxa are significant within ecosystem multifunctionality, as certain species fulfil essential functions such as recycling soil nutrients, promoting plant growth, influencing biogeochemical processes, and contributing to human health maintenance. However, there are still gaps regarding the relationship between microbial communities in volcanic rhizospheric soil and ecosystem multifunctionality. As a result, in this research, we employed Illumina MiSeq high-throughput sequencing to analyse the microbial community composition of rhizospheric soil from volcanic S. viridis. Compared with non-volcanic areas, volcanic soils have higher fungal alpha diversity and the absolute abundance of bacteria (16S gene copies) showed significant variation between the two successions (P < 0.0001). The network analysis further demonstrated that the microbial diversity in non-volcanic regions surpassed that of the volcanic area. The volcanic fungi network has more nodes and edges, is more complex than non-volcanic areas (Nodes: 425 vs. 770; Edges: 21844 vs. 74532), and more rhizosphere growth-promoting bacteria are enriched. Regression analysis and correlation networks showed that fungal communities were more closely associated with ecosystem multifunctionality than bacteria. This study lays the groundwork for examining the microbial keystone taxa in the rhizosphere of volcanic plants and offers valuable insights into the multifaceted functions of plant rhizospheric soil ecosystems.
关键种在生态系统多功能性中具有重要意义，因为某些物种履行着重要的功能，如循环利用土壤养分、促进植物生长、影响生物地球化学过程以及有助于维护人类健康。然而，关于火山根际土壤中的微生物群落与生态系统多功能性之间的关系，仍存在一些空白。因此，在这项研究中，我们采用 Illumina MiSeq 高通量测序来分析来自火山 S. viridis 的根际土壤的微生物群落组成。与非火山地区相比，火山土壤的真菌 alpha 多样性更高，细菌（16S 基因拷贝）的绝对丰度在两个演替中存在显著差异（P<0.0001）。网络分析进一步表明，非火山地区的微生物多样性超过了火山地区。非火山地区的细菌网络具有更多的节点和边，比火山地区更复杂（节点：425 对 770；边：21844 对 74532），并且富集了更多的根际促生细菌。回归分析和相关网络表明，真菌群落与生态系统多功能性的关联比细菌更为密切。本研究为研究火山植物根际的微生物关键种奠定了基础，并为植物根际土壤生态系统的多方面功能提供了有价值的见解。
