Zhou Yingying, Jiang Pan, Ding Yuanyuan, Zhang Yuping, Yang Sha, Liu Xinhua, Cao Chunxin, Luo Gongwen, Ou Lijun
Hunan Agricultural University, Changsha, 410128, China.
Hunan Key Laboratory of Vegetable Biology, Changsha, 410128, China.
Microb Ecol. 2025 Jan 31;88(1):1. doi: 10.1007/s00248-025-02499-z.
Exploring endospheric and rhizospheric microbiomes and their associations can help us to understand the pathological status of capsicum (Capsicum annuum L.) for implementing appropriate management strategies. To elucidate the differences among plants with distinct pathological status in the communities and functions of the endospheric and rhizospheric microbiomes, the samples of healthy and diseased capsicum plants, along with their rhizosphere soils, were collected from a long-term cultivation field. The results indicated a higher bacterial richness in the healthy rhizosphere than in the diseased rhizosphere (P < 0.05), with rhizospheric bacterial diversity surpassing endospheric bacterial diversity. The community assemblies of both the endospheric and rhizospheric microbiomes were driven by a combination of stochastic and deterministic processes, with the stochastic processes playing a primary role. The majority of co-enriched taxa in the healthy endophyte and rhizosphere mainly belonged to bacterial Proteobacteria, Actinobacteria, and Firmicutes, as well as fungal Ascomycota. Most of the bacterial indicators, primarily Alphaproteobacteria and Actinobacteria, were enriched in the healthy rhizosphere, but not in the diseased rhizosphere. In addition, most of the fungal indicators were enriched in both the healthy and diseased endosphere. The diseased endophyte constituted a less complex and stable microbial community than the healthy endophyte, and meanwhile, the diseased rhizosphere exhibited a higher complexity but lower stability than the healthy rhizosphere. Notably, only a microbial function, namely biosynthesis of other secondary metabolites, was higher in the healthy endophytes than in the diseased endophyte. These findings indicated the distinct responses of rhizospheric and endospheric microbiomes to capsicum pathological status, and in particular, provided a new insight into leveraging soil and plant microbial resources to enhance agriculture production.
探索辣椒（Capsicum annuum L.）的内生菌和根际微生物群落及其关联，有助于我们了解辣椒的病理状况，从而实施适当的管理策略。为了阐明具有不同病理状况的植物在其内生菌和根际微生物群落结构与功能上的差异，从一个长期种植田采集了健康和患病辣椒植株样本及其根际土壤。结果表明，健康根际的细菌丰富度高于患病根际（P < 0.05），根际细菌多样性超过内生细菌多样性。内生菌和根际微生物群落的组装受随机过程和确定性过程共同驱动，其中随机过程起主要作用。健康内生菌和根际中共同富集的大多数分类群主要属于细菌变形菌门、放线菌门和厚壁菌门，以及真菌子囊菌门。大多数细菌指示菌，主要是α-变形菌纲和放线菌，在健康根际中富集，但在患病根际中未富集。此外，大多数真菌指示菌在健康和患病的内生菌中均有富集。患病内生菌构成的微生物群落比健康内生菌更简单、稳定性更低，同时，患病根际比健康根际表现出更高的复杂性但更低的稳定性。值得注意的是，只有一种微生物功能，即其他次生代谢产物的生物合成，在健康内生菌中比患病内生菌中更高。这些发现表明根际和内生微生物群落对辣椒病理状况有不同反应，特别是为利用土壤和植物微生物资源提高农业产量提供了新的见解。
