Daoust R, Morais R
Chem Biol Interact. 1986 Jan;57(1):55-64. doi: 10.1016/0009-2797(86)90048-7.
Degenerative and regenerative changes induced in rat liver by single exposure to diethylnitrosamine (DEN) were examined by morphological and biochemical approaches. Apoptotic changes were observed in livers of rats exposed to a 'subnecrogenic' dose of DEN (10 mg/kg) as well as in liver parenchyma of those receiving a necrogenic dose (100 mg/kg). Zonal centrilobular necrosis was observed exclusively in the latter group. Regenerative changes, i.e., increases in DNA synthesis, labeling index and mitotic activity, occurred only in animals exposed to the higher dose. The mitogenic effect obtained in these conditions was about half that induced by two-thirds hepatectomy and the maximum response occurred about 24 h later than in partially hepatectomized rats.
通过形态学和生物化学方法研究了单次暴露于二乙基亚硝胺（DEN）后大鼠肝脏中诱导的退行性和再生性变化。在暴露于“亚致死剂量”DEN（10mg/kg）的大鼠肝脏以及接受致死剂量（100mg/kg）的大鼠肝脏实质中均观察到凋亡变化。仅在后一组中观察到小叶中心性带状坏死。再生性变化，即DNA合成、标记指数和有丝分裂活性增加，仅发生在暴露于较高剂量的动物中。在这些条件下获得的促有丝分裂作用约为三分之二肝切除诱导作用的一半，最大反应比部分肝切除大鼠晚约24小时出现。
