Kuick R, Boerwinkle E, Hanash S M, Sing C F
Comput Biomed Res. 1986 Feb;19(1):90-102. doi: 10.1016/0010-4809(86)90009-1.
For the development of valid algorithms for matching protein spots between two-dimensional gels, it is essential that one has an understanding of the relative roles of the many sources of variability affecting the location of spots. We first consider the contribution of observers to the measurement variability of spot location, arriving at a simple model for these effects. Next we present an analysis of the variability in spot locations for a sample of gels containing duplicate gels for each sample. Our data indicate that both differences between duplicates and between samples are considerable, and that the size of the effects depends on the region of the gel being considered. In the discussion we examine several matching strategies that match large groups of gels based on algorithms which match two gels at a time.
为了开发用于匹配二维凝胶之间蛋白质斑点的有效算法，必须了解影响斑点位置的多种变异性来源的相对作用。我们首先考虑观察者对斑点位置测量变异性的贡献，得出这些影响的一个简单模型。接下来，我们对包含每个样品重复凝胶的凝胶样品的斑点位置变异性进行分析。我们的数据表明，重复凝胶之间以及样品之间的差异都相当大，并且影响的大小取决于所考虑的凝胶区域。在讨论中，我们研究了几种基于一次匹配两个凝胶的算法来匹配大量凝胶的匹配策略。
